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LF elisa kit :: Human Lactoferrin ELISA Kit

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Catalog # MBS733033
Unit / Price
  48-Strip-Wells-(Competitive)  /  $440 +1 FREE 8GB USB
  48-Strip-Wells-(Sandwich)  /  $440 +1 FREE 8GB USB
  96-Strip-Wells-(Competitive)  /  $640 +1 FREE 8GB USB
  96-Strip-Wells-(Sandwich)  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells-(Competitive)  /  $2,895 +3 FREE 8GB USB
  5x96-Strip-Wells-(Sandwich)  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells-(Competitive)  /  $5,415 +6 FREE 8GB USB
  10x96-Strip-Wells-(Sandwich)  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Lactoferrin (LF), ELISA Kit

Popular Item
Full Product Name

Human Lactoferrin ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Competitive ELISA Manual Insert
Competitive ELISA PDF Manual
Sandwich ELISA Manual Insert
Sandwich ELISA PDF Manual
Request for Current Manual Insert
OMIM
gene 245480
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Detection Range
250-5000 pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forLFpurchase
MBS733033 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lactoferrin (LF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LF. The ELISA analytical biochemical technique of the MBS733033 kit is based on LF antibody-LF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LF elisa kit
Intended Uses: This LTF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LTF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: LTF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LTF. Standards or samples are then added to the microtiter plate wells and LTF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LTF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for LTF are added to each well to "sandwich" the LTF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LTF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LTF concentration in each sample is interpolated from this standard curve.
Product Categories/Family for LF elisa kit

Typical Testing Data/Standard Curve (for reference only) of LF elisa kit
LF elisa kit Typical Testing Data/Standard Curve (for reference only) image
NCBI/Uniprot data below describe general gene information for LF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
73,161 Da
NCBI Official Full Name
lactoferrin
NCBI Official Synonym Full Names
lactotransferrin
NCBI Official Symbol
NCBI Official Synonym Symbols
LF; HLF2; GIG12; HEL110
  [Similar Products]
NCBI Protein Information
lactotransferrin; talalactoferrin; neutrophil lactoferrin; growth-inhibiting protein 12; epididymis luminal protein 110
UniProt Protein Name
Lactotransferrin
UniProt Synonym Protein Names
Growth-inhibiting protein 12; TalalactoferrinCleaved into the following 5 chains:Lactoferricin-H; Lfcin-H; Kaliocin-1; Lactoferroxin-A; Lactoferroxin-B; Lactoferroxin-C
UniProt Gene Name
UniProt Synonym Gene Names
GIG12; LF; Lactoferrin; Lfcin-H  [Similar Products]
UniProt Entry Name
TRFL_HUMAN
NCBI Summary for LF
This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
UniProt Comments for LF
LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Secreted; EC 3.4.21.-; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: extracellular space; specific granule; cytoplasm; extracellular region; nucleus; secretory granule

Molecular Function: heparin binding; protein serine/threonine kinase activator activity; protein binding; DNA binding; ferric iron binding; serine-type endopeptidase activity; iron ion binding

Biological Process: negative regulation of lipopolysaccharide-mediated signaling pathway; interaction with host; ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; positive regulation of toll-like receptor 4 signaling pathway; antifungal humoral response; proteolysis; iron ion transport; humoral immune response; activation of NF-kappaB transcription factor; regulation of cytokine production; positive regulation of osteoblast differentiation; retinal homeostasis; negative regulation of viral genome replication; innate immune response in mucosa; response to host immune response; positive regulation of osteoblast proliferation; antibacterial humoral response; regulation of tumor necrosis factor production; iron assimilation by chelation and transport; negative regulation of viral reproduction; negative regulation of ATPase activity; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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