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LGALS3 recombinant protein :: Galectin-3 Recombinant Protein

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Catalog # MBS691554
Unit / Price
  0.01 mg  /  $200 +1 FREE 8GB USB
  0.05 mg  /  $340 +1 FREE 8GB USB
LGALS3 recombinant protein
Product Name

Galectin-3 (LGALS3), Recombinant Protein

Popular Item
Also Known As

Human Galectin-3

Product Synonym Names
Recombinant Human Galectin-3
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
250
Sequence
ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPG QAPP GAYPGQAPPG AYHGAPGAYP GAPAPGVYPG PPSGPGAYPS S GQPSAPGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTV KPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GR EERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLN EI SKLGISGDID LTSASYTMI
Chromosome Location
Chromosome: 14; NC_000014.9 (55129217..55145430). Location: 14q22.3
OMIM
153619
3D Structure
ModBase 3D Structure for P17931
Host
E Coli
Species Reactivity
Human
Purity/Purification
> 98% by SDS-PAGE and HPLC analysis
Form/Format
Lyophilized from 0.5x PBS, pH 7.2
Endotoxin Level
< 0.1 ng per ug of Galectin-3
Biological Activity
Determined by its ability to chemoattract human blood monocytes. Chemotactic activity was observed at a concentration of 2.5 ug/ml with a peak response obtained at 250 ug/ml.
Reconstitution
The human Galectin-3 should be reconstituted in water to a concentration of 0.1-1.0 mg/ml. Do not vortex! This solution can be diluted in water or other buffer solutions or stored at -20 degree C.
Preparation and Storage
The lyophilized protein is stable for a 1 month at room temperature or 6 months at 4 degree C. The reconstituted protein is stable for 1 week at 2 degree C to 8 degree C and 3 months at -20 degreeC to 8 degree c and 3 months at -20 degree C to -80 degree C. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (e.g. 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of LGALS3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
LGALS3 recombinant protein
Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with beta-galactoside moieties. Galectin-3 regulates a number of biological processes, including embryogenesis, inflammatory responses, cell progression and metastasis. Galectin-3 is normally expressed in epithelia of a variety of tissues, including colon and endometrium, and in various inflammatory cells, including macrophages. Galectin-3 can function intracellularly, in controlling cell cycle and preventing T-cell apoptosis, and also extracellularly, in activating various cells, including monocytes/macrophages, mast cells, neutrophils, and lymphocytes. Expression of Galectin-3 is affected by neoplastic transformation, being up-regulated in certain types of lymphomas, and in thyroid and hepatic carcinomas. Conversely, it is down-regulated in other cancers, such as colon, breast, ovarian, and uterine. Recombinant human Galectin-3 is a globular 26.0 kDa protein containing 250 amino acid residues, but no disulfide bonds.
NCBI/Uniprot data below describe general gene information for LGALS3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
NCBI Official Full Name
galectin-3 isoform 1
NCBI Official Synonym Full Names
lectin, galactoside-binding, soluble, 3
NCBI Official Symbol
LGALS3  [Similar Products]
NCBI Official Synonym Symbols
L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
  [Similar Products]
NCBI Protein Information
galectin-3; lectin L-29; 35 kDa lectin; MAC-2 antigen; IgE-binding protein; laminin-binding protein; galactose-specific lectin 3; carbohydrate-binding protein 35
UniProt Protein Name
Galectin-3
UniProt Synonym Protein Names
35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen
Protein Family
UniProt Gene Name
LGALS3  [Similar Products]
UniProt Synonym Gene Names
MAC2; Gal-3; CBP 35; GALBP  [Similar Products]
UniProt Entry Name
LEG3_HUMAN
NCBI Summary for LGALS3
This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]
UniProt Comments for LGALS3
Function: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis

By similarity. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Ref.15 Ref.16 Ref.18

Subunit structure: Probably forms homo- or heterodimers. Interacts with DMBT1

By similarity. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA. Ref.14 Ref.15

Subcellular location: Cytoplasm. Nucleus. Secreted. Note: Secreted by a non-classical secretory pathway and associates with the cell surface. Ref.5 Ref.15 Ref.18

Tissue specificity: A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes. Ref.17

Sequence similarities: Contains 1 galectin domain.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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