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LMNB1 elisa kit :: Human Lamin B1 (LMNB1) ELISA Kit

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Catalog # MBS2024959 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $270 +1 FREE 8GB USB
  48-Strip-Wells  /  $505 +1 FREE 8GB USB
  96-Strip-Wells  /  $685 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,770 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,025 +6 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

Lamin B1 (LMNB1), ELISA Kit

Popular Item
Also Known As

Lamin B1 (LMNB1) ELISA Kit

Product Synonym Names
LMN; LMN2; LMNB
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
586
OMIM
150340
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Lamin B1 (LMNB1).
No significant cross-reactivity or interference between Lamin B1 (LMNB1) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.057ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Lamin B1 (LMNB1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Lamin B1 (LMNB1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LMNB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forLMNB1purchase
MBS2024959 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lamin B1 (LMNB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LMNB1. The ELISA analytical biochemical technique of the MBS2024959 kit is based on LMNB1 antibody-LMNB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LMNB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LMNB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LMNB1 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lamin B1 (LMNB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lamin B1 (LMNB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lamin B1 (LMNB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lamin B1 (LMNB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for LMNB1 elisa kit

Typical Standard Curve/Testing Data of LMNB1 elisa kit
LMNB1 elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS2024959. Click to request current manual
NCBI/Uniprot data below describe general gene information for LMNB1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
66,408 Da
NCBI Official Full Name
lamin B1
NCBI Official Synonym Full Names
lamin B1
NCBI Official Symbol
LMNB1  [Similar Products]
NCBI Official Synonym Symbols
LMN; ADLD; LMN2; LMNB
  [Similar Products]
NCBI Protein Information
lamin-B1
UniProt Protein Name
Lamin-B1
Protein Family
UniProt Gene Name
LMNB1  [Similar Products]
UniProt Synonym Gene Names
LMN2; LMNB  [Similar Products]
UniProt Entry Name
LMNB1_HUMAN
NCBI Summary for LMNB1
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq, Oct 2010]
UniProt Comments for LMNB1
Lamin B1: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. B-type lamins are phosphorylated within minutes of engaging the IgM surface receptor of resting splenic B cells. Nuclear lamins are cleaved by caspases during apoptosis.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 5q23.2

Cellular Component: nucleoplasm; nuclear membrane; membrane; lamin filament; nuclear inner membrane; nuclear envelope

Molecular Function: phospholipase binding; structural molecule activity

Biological Process: apoptosis; cell structure disassembly during apoptosis

Disease: Leukodystrophy, Demyelinating, Adult-onset, Autosomal Dominant
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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