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LMX1B elisa kit :: Chicken LIM/homeobox protein LMX-1.2 ELISA Kit

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Catalog # MBS288349
Unit / Price
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  48-Strip-Wells  /  $400 +1 FREE 8GB USB
  96-Strip-Wells  /  $530 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,305 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $4,250 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

LIM/homeobox protein LMX-1.2 (LMX1B), ELISA Kit

Full Product Name

Chicken LIM/homeobox protein LMX-1.2 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for P53413
Species Reactivity
Specificity
This assay recognizes recombinant and natural Chicken Homeobox protein LMX-1. No significant cross-reactivity or interference was observed.
Samples
Serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Assay Type
Competitive
Detection Range
0.78-50.0 ng/ml.
Sensitivity
The minimum detectable dose of Chicken Homeobox protein LMX-1 is typically less than 0.39 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero.
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LMX1B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for LMX1Bpurchase
MBS288349 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the LIM/homeobox protein LMX-1.2 (LMX1B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LMX1B. The ELISA analytical biochemical technique of the MBS288349 kit is based on LMX1B antibody-LMX1B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LMX1B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LMX1B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LMX1B elisa kit
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Homeobox protein LMX-1, During the reaction, Homeobox protein LMX-1 in the sample or standard competes with a fixed amount of biotin-labeled Homeobox protein LMX-1 for sites on a pre-coated Monoclonal antibody specific to Homeobox protein LMX-1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Homeobox protein LMX-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of LMX1B elisa kit
LMX1B elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS288349. Click to request current manual
NCBI/Uniprot data below describe general gene information for LMX1B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
45,027 Da
NCBI Official Full Name
LIM/homeobox protein LMX-1.2
NCBI Official Synonym Full Names
LIM homeobox transcription factor 1, beta
NCBI Official Symbol
LMX1B  [Similar Products]
NCBI Official Synonym Symbols
LMX; LMX1
  [Similar Products]
NCBI Protein Information
LIM/homeobox protein LMX-1.2
UniProt Protein Name
LIM/homeobox protein LMX-1.2
Protein Family
UniProt Gene Name
LMX1B  [Similar Products]
UniProt Synonym Gene Names
LMX; LMX1; Homeobox protein LMX-1; LIM/homeobox protein 1; LMX  [Similar Products]
UniProt Entry Name
LMX1B_CHICK
UniProt Comments for LMX1B
Specifies dorsal cell fate during limb development.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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