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LOX-12 elisa kit :: Chicken 12-Lipoxygenase ELISA Kit

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Catalog # MBS742482
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

12-Lipoxygenase (LOX-12), ELISA Kit

Full Product Name

Chicken 12-Lipoxygenase ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 17; NC_000017.10 (4534214..4544960, complement). Location: 17p13.3
3D Structure
ModBase 3D Structure for Q12938
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of LOX12. No significant cross-reactivity or interference between LOX12 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LOX12 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LOX-12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for LOX-12purchase
MBS742482 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 12-Lipoxygenase (LOX-12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LOX-12. The ELISA analytical biochemical technique of the MBS742482 kit is based on LOX-12 antibody-LOX-12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LOX-12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LOX-12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LOX-12 elisa kit
Intended Uses: This LOX12 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken LOX12. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: LOX12 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-LOX12 antibody and an LOX12-HRP conjugate. The assay sample and buffer are incubated together with LOX12-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LOX12 concentration since LOX12 from samples and LOX12-HRP conjugate compete for the anti-LOX12 antibody binding site. Since the number of sites is limited, as more sites are occupied by LOX12 from the sample, fewer sites are left to bind LOX12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LOX12 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for LOX-12 elisa kit

Typical Standard Curve/Testing Data of LOX-12 elisa kit
LOX-12 elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS742482. Click to request current manual
NCBI/Uniprot data below describe general gene information for LOX-12. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
9,448 Da
NCBI Official Full Name
12-lipoxygenase, partial
NCBI Official Synonym Full Names
arachidonate 15-lipoxygenase
NCBI Official Symbol
ALOX15  [Similar Products]
NCBI Official Synonym Symbols
15LOX-1; 15-LOX-1
  [Similar Products]
NCBI Protein Information
arachidonate 15-lipoxygenase; 15-LOX; 15-lipooxygenase-1; arachidonate omega-6 lipoxygenase
UniProt Protein Name
12-lipoxygenase
UniProt Entry Name
Q12938_HUMAN
UniProt Comments for LOX-12
ALOX15: Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. Belongs to the lipoxygenase family.

Protein type: EC 1.13.11.31; Oxidoreductase; EC 1.13.11.33; Motility/polarity/chemotaxis; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: extrinsic to internal side of plasma membrane; membrane; plasma membrane; lipid particle; cytosol

Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; hepoxilin A3 synthase activity; arachidonate 12-lipoxygenase activity; arachidonate 15-lipoxygenase activity; hepoxilin-epoxide hydrolase activity; iron ion binding

Biological Process: ossification; positive regulation of heterotypic cell-cell adhesion; wound healing; phosphatidylethanolamine biosynthetic process; leukotriene metabolic process; bone mineralization; lipoxygenase pathway; positive regulation of actin filament polymerization; negative regulation of adaptive immune response; arachidonic acid metabolic process; hepoxilin biosynthetic process; inflammatory response; apoptotic cell clearance
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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