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LOX-15 elisa kit :: Rat 15-Lipoxygenase ELISA Kit

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Catalog # MBS720787
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

15-Lipoxygenase (LOX-15), ELISA Kit

Full Product Name

Rat 15-Lipoxygenase ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
152392
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of LOX-15. No significant cross-reactivity or interference between LOX-15 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LOX-15 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
2.5-50ng/mL
Sensitivity
0.1ng/ml
Intended Uses
This LOX-15 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat LOX-15. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LOX-15 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forLOX-15purchase
MBS720787 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 15-Lipoxygenase (LOX-15) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LOX-15. The ELISA analytical biochemical technique of the MBS720787 kit is based on LOX-15 antibody-LOX-15 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LOX-15 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LOX-15. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LOX-15 elisa kit
Principle of the assay: LOX-15 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LOX-15 antibody and an LOX-15-HRP conjugate. The assay sample and buffer are incubated together with LOX-15-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LOX-15 concentration since LOX-15 from samples and LOX-15-HRP conjugate compete for the anti-LOX-15 antibody binding site. Since the number of sites is limited, as more sites are occupied by LOX-15 from the sample, fewer sites are left to bind LOX-15-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LOX-15 concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of LOX-15 elisa kit
LOX-15 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS720787. Click to request current manual
NCBI/Uniprot data below describe general gene information for LOX-15. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
74,804 Da
NCBI Official Full Name
15-lipoxygenase
NCBI Official Synonym Full Names
arachidonate 15-lipoxygenase
NCBI Official Symbol
ALOX15  [Similar Products]
NCBI Official Synonym Symbols
12-LOX; 15LOX-1; 15-LOX-1
  [Similar Products]
NCBI Protein Information
arachidonate 15-lipoxygenase; 15-LOX; 12/15-lipoxygenase; 15-lipooxygenase-1; arachidonate omega-6 lipoxygenase; arachidonate 12-lipoxygenase, leukocyte-type
UniProt Protein Name
Arachidonate 15-lipoxygenase
UniProt Synonym Protein Names
12/15-lipoxygenase; Arachidonate 12-lipoxygenase, leukocyte-type (EC:1.13.11.31); 12-LOX; Arachidonate omega-6 lipoxygenase
UniProt Gene Name
ALOX15  [Similar Products]
UniProt Synonym Gene Names
LOG15; 15-LOX; 15-LOX-1; 12-LOX  [Similar Products]
UniProt Entry Name
LOX15_HUMAN
UniProt Comments for LOX-15
ALOX15: Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. Belongs to the lipoxygenase family.

Protein type: Lipid Metabolism - arachidonic acid; EC 1.13.11.31; EC 1.13.11.33; Lipid Metabolism - linoleic acid; Motility/polarity/chemotaxis; Oxidoreductase

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: extrinsic to internal side of plasma membrane; membrane; plasma membrane; lipid particle; cytosol

Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; protein binding; arachidonate 12-lipoxygenase activity; hepoxilin A3 synthase activity; arachidonate 15-lipoxygenase activity; hepoxilin-epoxide hydrolase activity; iron ion binding

Biological Process: ossification; positive regulation of heterotypic cell-cell adhesion; lipoxygenase pathway; wound healing; positive regulation of actin filament polymerization; negative regulation of adaptive immune response; phosphatidylethanolamine biosynthetic process; arachidonic acid metabolic process; leukotriene metabolic process; hepoxilin biosynthetic process; inflammatory response; apoptotic cell clearance; bone mineralization
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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