View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List LOX elisa kit :: Canine Lysyl oxidase ELISA Kit Catalog # MBS750232 Unit / Price 48-Strip-Wells / $495 +1 FREE 8GB USB 96-Strip-Wells / $700 +1 FREE 8GB USB 5x96-Strip-Wells / $3,105 +3 FREE 8GB USB 10x96-Strip-Wells / $5,865 +7 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Lysyl oxidase (LOX), ELISA Kit Full Product Name Canine Lysyl oxidase ELISA Kit Product Gene Name LOX elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual OMIM 153455 Species Reactivity Canine Samples Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate Assay Type Competitive Sensitivity 0.1 ng/mL. Preparation and Storage Store all reagents at 2-8 degree C. Sample Preparation We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team ISO Certification Manufactured in an ISO 13485:2016 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of LOX elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for LOX purchase MBS750232 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lysyl oxidase (LOX) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LOX. The ELISA analytical biochemical technique of the MBS750232 kit is based on LOX antibody-LOX antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LOX antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LOX. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for LOX elisa kit Intended Uses: This LOX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine LOX. This ELISA kit for research use only!Principle of the Assay: LOX ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LOX antibody and an LOX-HRP conjugate. The assay sample and buffer are incubated together with LOX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LOX concentration since LOX from samples and LOX-HRP conjugate compete for the anti-LOX antibody binding site. Since the number of sites is limited, as more sites are occupied by LOX from the sample, fewer sites are left to bind LOX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LOX concentration in each sample is interpolated from this standard curve. Sample Manual Insert of MBS750232. NCBI/Uniprot data below describe general gene information for LOX. It may not necessarily be applicable to this product. NCBI GI # 257065 NCBI GeneID 4015 UniProt Secondary Accession # Q5FWF0; B2R5Q3[Other Products] UniProt Related Accession # P28300[Other Products] Molecular Weight 46,944 Da TOP NCBI Official Full Name lysyl oxidase NCBI Official Synonym Full Names lysyl oxidase NCBI Official Symbol LOX [Similar Products] NCBI Protein Information protein-lysine 6-oxidase UniProt Protein Name Protein-lysine 6-oxidase UniProt Synonym Protein Names Lysyl oxidase Protein Family Lysyl oxidase UniProt Gene Name LOX [Similar Products] UniProt Entry Name LYOX_HUMAN TOP NCBI Summary for LOX The protein encoded by this gene is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, the encoded protein may have a role in tumor suppression. Defects in this gene are a cause of autosomal recessive cutis laxa type I (CL type I). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010] TOP UniProt Comments for LOX LOX: Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. Belongs to the lysyl oxidase family.Protein type: Secreted, signal peptide; EC 1.4.3.13; Secreted; OxidoreductaseChromosomal Location of Human Ortholog: 5q23.2Cellular Component: proteinaceous extracellular matrix; extracellular space; collagen; extracellular region; nucleusMolecular Function: protein binding; copper ion binding; protein-lysine 6-oxidase activityBiological Process: response to drug; elastic fiber assembly; blood vessel development; extracellular matrix organization and biogenesis; collagen fibril organization; wound healing; response to steroid hormone stimulus; protein modification process; lung developmentDisease: Cutis Laxa, Autosomal Recessive, Type Ia Research Articles on LOX 1. LOX, LOXL1 and LOXL2 are downstream PPARgamma targets and that LOX activity is a negative regulator of trophoblastic cell invasion TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP Products associated with LOX elisa kit Reference Product PubMed Publications ALOX5 elisa kit >356 publications with LOX and ALOX5 SRC elisa kit >30 publications with LOX and SRC FGF2 elisa kit >13 publications with LOX and FGF2 FBLN5 elisa kit >7 publications with LOX and FBLN5 FBN1 elisa kit >4 publications with LOX and FBN1 TGFBI elisa kit >2 publications with LOX and TGFBI FN1 elisa kit >2 publications with LOX and FN1 ELN elisa kit >1 publications with LOX and ELN Pathways associated with LOX elisa kit Products by Pathway Pathway Diagram Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram Collagen Formation Pathway antibodies Collagen Formation Pathway Diagram Crosslinking Of Collagen Fibrils Pathway antibodies Crosslinking Of Collagen Fibrils Pathway Diagram Elastic Fibre Formation Pathway antibodies Elastic Fibre Formation Pathway Diagram Extracellular Matrix Organization Pathway antibodies Extracellular Matrix Organization Pathway Diagram Diseases associated with LOX elisa kit Disease Name Pubmed Publications Inflammation Antibodies >558 publications with LOX and Inflammation Cardiovascular Diseases Antibodies >547 publications with LOX and Cardiovascular Diseases Disease Models, Animal Antibodies >360 publications with LOX and Disease Models, Animal Necrosis Antibodies >220 publications with LOX and Necrosis Heart Diseases Antibodies >171 publications with LOX and Heart Diseases Neoplasms, Experimental Antibodies >149 publications with LOX and Neoplasms, Experimental Brain Diseases Antibodies >148 publications with LOX and Brain Diseases Hypertension Antibodies >133 publications with LOX and Hypertension Fibrosis Antibodies >119 publications with LOX and Fibrosis Lung Neoplasms Antibodies >115 publications with LOX and Lung Neoplasms Organs/Tissues associated with LOX elisa kit Organ/Tissue Name Pubmed Publications Vascular Antibodies >684 publications with LOX and Vascular Muscle Antibodies >376 publications with LOX and Muscle Lung Antibodies >267 publications with LOX and Lung Heart Antibodies >244 publications with LOX and Heart Liver Antibodies >231 publications with LOX and Liver Bone Antibodies >213 publications with LOX and Bone Skin Antibodies >173 publications with LOX and Skin Kidney Antibodies >157 publications with LOX and Kidney Embryonic Tissue Antibodies >153 publications with LOX and Embryonic Tissue Prostate Antibodies >119 publications with LOX and Prostate TOP