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Add To Compare List Lp-PLA2 elisa kit :: Rat lipoprotein-associated phospholipase A2, Lp-PLA2 ELISA Kit Catalog # MBS704213 Unit / Price 24-Strip-Wells (LIMIT 1) / $285 +1 FREE 8GB USB 48-Strip-Wells / $490 +1 FREE 8GB USB 96-Strip-Wells / $685 +1 FREE 8GB USB 5x96-Strip-Wells / $2,845 +3 FREE 8GB USB 10x96-Strip-Wells / $5,435 +6 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name lipoprotein-associated phospholipase A2, Lp-PLA2, ELISA Kit Full Product Name Rat lipoprotein-associated phospholipase A2, Lp-PLA2 ELISA Kit Product Synonym Names Rat lipoprotein-associated phospholipase A2; Lp-PLA2 ELISA Kit; lipoprotein-associated phospholipase A2; Lp-PLA2 Product Gene Name Lp-PLA2 elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual MBS704213 Testing Data Testing Data PDF Species Reactivity Rat Detection Range 7.8 ng/ml-500 ng/ml Sensitivity 2 ng/ml Detection Wavelength 450 nm Sample Volume 50-100ul Preparation and Storage Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions. ISO Certification Manufactured in an ISO 9001:2015 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of Lp-PLA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for Lp-PLA2 purchase MBS704213 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the lipoprotein-associated phospholipase A2, Lp-PLA2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Lp-PLA2. The ELISA analytical biochemical technique of the MBS704213 kit is based on Lp-PLA2 antibody-Lp-PLA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Lp-PLA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Lp-PLA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for Lp-PLA2 elisa kit Introduction: Lipoprotein-associated phospholipase A2 (Lp-PLA2, also known as platelet activating factor acetyl-hydrolase), is a relatively recent marker that has emerged as a major, independent predictor of CHD. Lp-PLA2 is an active phospholipase that circulates in plasma bound to LDL, and it appears to act almost exclusively on oxidized LDL to generate lysophosphatidyl-choline, which is proinflammatory and is considered to be atherogenic. Thus, Lp-PLA2 represents an interesting link between lipoproteinoxidation and vascular inflammation, which likely explain its strong, independent association with CHD risk. Data from the recent ARIC study have indicated that the predictive power of Lp-PLA2 is attenuated after adjustment for multiple established risk factors, but remains strong in the sub-group with LDLcholesterol levels of < 130 mg/dL. Moreover, this study indicated that there was an interaction between Lp-PLA2 and CRP levels, and the strongest predictor for CHD risk (hazard ratio = 2.95) was found for the combination of high CRP (> 3 mg/L) and high Lp-PLA2 (= 422 ug/L) in the presence of LDL < 130 mg/dL. Therefore, CRP and Lp- PLA2 appear to be particularly useful in predicting CHD risk in the presence of LDL cholesterol in the normal range. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lp-PLA2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Lp-PLA2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Lp-PLA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Lp-PLA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Typical Testing Data/Standard Curve (for reference only) of Lp-PLA2 elisa kit TOP Sample Manual Insert of MBS704213. Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP