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Lp-PLA2 elisa kit :: Rat lipoprotein-associated phospholipase A2, Lp-PLA2 ELISA Kit

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Catalog # MBS704213
Unit / Price
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  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $435 +1 FREE 8GB USB
  96-Strip-Wells  /  $625 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

lipoprotein-associated phospholipase A2, Lp-PLA2, ELISA Kit

Full Product Name

Rat lipoprotein-associated phospholipase A2, Lp-PLA2 ELISA Kit

Product Synonym Names
Rat lipoprotein-associated phospholipase A2; Lp-PLA2 ELISA Kit; lipoprotein-associated phospholipase A2; Lp-PLA2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS704213 Testing Data
Species Reactivity
Detection Range
7.8 ng/ml-500 ng/ml
Sensitivity
2 ng/ml
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Lp-PLA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Lp-PLA2purchase
MBS704213 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the lipoprotein-associated phospholipase A2, Lp-PLA2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Lp-PLA2. The ELISA analytical biochemical technique of the MBS704213 kit is based on Lp-PLA2 antibody-Lp-PLA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Lp-PLA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Lp-PLA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Lp-PLA2 elisa kit
Introduction: Lipoprotein-associated phospholipase A2 (Lp-PLA2, also known as platelet activating factor acetyl-hydrolase), is a relatively recent marker that has emerged as a major, independent predictor of CHD. Lp-PLA2 is an active phospholipase that circulates in plasma bound to LDL, and it appears to act almost exclusively on oxidized LDL to generate lysophosphatidyl-choline, which is proinflammatory and is considered to be atherogenic. Thus, Lp-PLA2 represents an interesting link between lipoproteinoxidation and vascular inflammation, which likely explain its strong, independent association with CHD risk. Data from the recent ARIC study have indicated that the predictive power of Lp-PLA2 is attenuated after adjustment for multiple established risk factors, but remains strong in the sub-group with LDLcholesterol levels of < 130 mg/dL. Moreover, this study indicated that there was an interaction between Lp-PLA2 and CRP levels, and the strongest predictor for CHD risk (hazard ratio = 2.95) was found for the combination of high CRP (> 3 mg/L) and high Lp-PLA2 (= 422 ug/L) in the presence of LDL < 130 mg/dL. Therefore, CRP and Lp- PLA2 appear to be particularly useful in predicting CHD risk in the presence of LDL cholesterol in the normal range.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lp-PLA2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Lp-PLA2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Lp-PLA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Lp-PLA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of Lp-PLA2 elisa kit
Lp-PLA2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS704213. Click to request current manual
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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