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LPAR3 elisa kit :: Human lysophosphatidic acid receptor 3 ELISA Kit

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Catalog # MBS939569
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Typical Testing Data/Standard Curve (for reference only)
Product Name

lysophosphatidic acid receptor 3 (LPAR3), ELISA Kit

Popular Item
Full Product Name

Human Lysophosphatidic acid receptor 3, LPAR3 ELISA Kit

Product Synonym Names
Human Lysophosphatidic acid receptor 3 (LPAR3) ELISA kit; EDG7; Edg-7; FLJ98231; GPCR; HOFNH30; LP-A3; LPA3; RP4-678I3; LPA receptor EDG7; OTTHUMP00000011573; calcium-mobilizing lysophosphatidic acid receptor LP-A3; endothelial cell differentiation gene 7; end; lysophosphatidic acid receptor 3
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS939569 Testing Data
OMIM
605106
3D Structure
ModBase 3D Structure for Q9UBY5
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human LPAR3. No significant cross-reactivity or interference between Human LPAR3 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
25 pg/ml-1600 pg/ml
Sensitivity
6.25 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LPAR3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for LPAR3purchase
MBS939569 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the lysophosphatidic acid receptor 3 (LPAR3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LPAR3. The ELISA analytical biochemical technique of the MBS939569 kit is based on LPAR3 antibody-LPAR3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LPAR3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LPAR3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LPAR3 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LPAR3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LPAR3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPAR3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPAR3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of LPAR3 elisa kit
LPAR3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS939569. Click to request current manual
NCBI/Uniprot data below describe general gene information for LPAR3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,128 Da
NCBI Official Full Name
lysophosphatidic acid receptor 3
NCBI Official Synonym Full Names
lysophosphatidic acid receptor 3
NCBI Official Symbol
LPAR3  [Similar Products]
NCBI Official Synonym Symbols
EDG7; GPCR; LPA3; Edg-7; LP-A3; HOFNH30; RP4-678I3
  [Similar Products]
NCBI Protein Information
lysophosphatidic acid receptor 3; LPA-3; LPA receptor 3; LPA receptor EDG7; lysophosphatidic acid receptor Edg-7; endothelial cell differentiation gene 7; calcium-mobilizing lysophosphatidic acid receptor LP-A3; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 7
UniProt Protein Name
Lysophosphatidic acid receptor 3
UniProt Synonym Protein Names
Lysophosphatidic acid receptor Edg-7
UniProt Gene Name
LPAR3  [Similar Products]
UniProt Synonym Gene Names
EDG7; LPA3; LPA receptor 3; LPA-3  [Similar Products]
UniProt Entry Name
LPAR3_HUMAN
NCBI Summary for LPAR3
This gene encodes a member of the G protein-coupled receptor family, as well as the EDG family of proteins. This protein functions as a cellular receptor for lysophosphatidic acid and mediates lysophosphatidic acid-evoked calcium mobilization. This receptor couples predominantly to G(q/11) alpha proteins. [provided by RefSeq, Jul 2008]
UniProt Comments for LPAR3
EDG7: Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. May play a role in the development of ovarian cancer. Seems to be coupled to the G(i)/G(o) and G(q) families of heteromeric G proteins. Belongs to the G-protein coupled receptor 1 family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 1p22.3

Cellular Component: axon; integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; G-protein alpha-subunit binding; phospholipid binding; lipid binding

Biological Process: synaptic transmission; G-protein signaling, coupled to cyclic nucleotide second messenger; elevation of cytosolic calcium ion concentration; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); activation of MAPK activity; bleb formation; positive regulation of calcium ion transport; positive regulation of collateral sprouting
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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