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LpPLA2 elisa kit :: Human Phospholipase A2, Lipoprotein Associated (LpPLA2) ELISA Kit

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Catalog # MBS2702623 (SPECIAL PROMOTIONAL PRICING for a limited time)
Unit / Price
  24-Strip-Wells  /  $210 +1 FREE 8GB USB
  48-Strip-Wells  /  $330 +1 FREE 8GB USB
  96-Strip-Wells  /  $440 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,800 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,345 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Phospholipase A2, Lipoprotein Associated (LpPLA2), ELISA Kit

Popular Item
Also Known As

Human Phospholipase A2, Lipoprotein Associated (LpPLA2) ELISA Kit

Product Synonym Names
PLA2G7; PAF-AH; PAFAH; Lp-PLA2; LDL-PLA2; Platelet Activating Factor Acetylhydrolase,Plasma; Phospholipase A2,Group VII; LDL-associated phospholipase A2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
144
OMIM
M22430 mRNA
3D Structure
ModBase 3D Structure for P14555
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Lipoprotein Associated (LpPLA2).
No significant cross-reactivity or interference between Phospholipase A2, Lipoprotein Associated (LpPLA2) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma and Other Biological Fluids
Detection Range
0.625-40ng/mL
Sensitivity
< 0.286ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Phospholipase A2, Lipoprotein Associated (LpPLA2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Phospholipase A2, Lipoprotein Associated (LpPLA2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LpPLA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forLpPLA2purchase
MBS2702623 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phospholipase A2, Lipoprotein Associated (LpPLA2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LpPLA2. The ELISA analytical biochemical technique of the MBS2702623 kit is based on LpPLA2 antibody-LpPLA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LpPLA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LpPLA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LpPLA2 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Lipoprotein Associated (LpPLA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Lipoprotein Associated (LpPLA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Lipoprotein Associated (LpPLA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2, Lipoprotein Associated (LpPLA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of LpPLA2 elisa kit
LpPLA2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2702623. Click to request current manual
NCBI/Uniprot data below describe general gene information for LpPLA2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
16,083 Da
NCBI Official Full Name
phospholipase A2, membrane associated
NCBI Official Synonym Full Names
phospholipase A2 group IIA
NCBI Official Symbol
PLA2G2A  [Similar Products]
NCBI Official Synonym Symbols
MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2
  [Similar Products]
NCBI Protein Information
phospholipase A2, membrane associated
UniProt Protein Name
Phospholipase A2, membrane associated
UniProt Synonym Protein Names
GIIC sPLA2; Group IIA phospholipase A2; Non-pancreatic secretory phospholipase A2; NPS-PLA2; Phosphatidylcholine 2-acylhydrolase 2A
UniProt Gene Name
PLA2G2A  [Similar Products]
UniProt Synonym Gene Names
PLA2B; PLA2L; RASF-A; NPS-PLA2  [Similar Products]
NCBI Summary for LpPLA2
The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009]
UniProt Comments for LpPLA2
Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides (PubMed:2925633). Thought to participate in the regulation of phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Independent of its catalytic activity, acts as a ligand for integrins (PubMed:18635536, PubMed:25398877). Binds to and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 (PubMed:18635536, PubMed:25398877). Binds to a site (site 2) which is distinct from the classical ligand-binding site (site 1) and induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:25398877). Induces cell proliferation in an integrin-dependent manner (PubMed:18635536).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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