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LRBA elisa kit :: Mouse LPS-responsive vesicle trafficking, beach and anchor containing ELISA Kit

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Catalog # MBS902174
Unit / Price DISCONTINUED
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Typical Testing Data/Standard Curve (for reference only)
Product Name

LPS-responsive vesicle trafficking, beach and anchor containing (LRBA), ELISA Kit

Full Product Name

Mouse Lipopolysaccharide-responsive and beige-like anchor protein, LRBA ELISA Kit

Product Synonym Names
Mouse Lipopolysaccharide-responsive and beige-like anchor protein (LRBA) ELISA kit; BGL; CDC4L; DKFZp686A09128; DKFZp686K03100; DKFZp686P2258; FLJ16600; FLJ25686; LAB300; LBA; MGC72098; cell division cycle 4-like; vesicle trafficking; beach and anchor containing; LPS-responsive vesicle trafficking; beach and anchor containing
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS902174 Testing Data
3D Structure
ModBase 3D Structure for Q9ESE1
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse LRBA. No significant cross-reactivity or interference between Mouse LRBA and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
31.25 pg/ml-2000 pg/ml
Sensitivity
7.81 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LRBA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forLRBApurchase
MBS902174 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the LPS-responsive vesicle trafficking, beach and anchor containing (LRBA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LRBA. The ELISA analytical biochemical technique of the MBS902174 kit is based on LRBA antibody-LRBA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LRBA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LRBA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LRBA elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LRBA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LRBA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRBA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRBA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of LRBA elisa kit
LRBA elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS902174. Click to request current manual
NCBI/Uniprot data below describe general gene information for LRBA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
317,064 Da
NCBI Official Full Name
lipopolysaccharide-responsive and beige-like anchor protein isoform gamma
NCBI Official Synonym Full Names
LPS-responsive beige-like anchor
NCBI Official Symbol
NCBI Official Synonym Symbols
Lba; C80285; Lab300; D3Ertd775e
  [Similar Products]
NCBI Protein Information
lipopolysaccharide-responsive and beige-like anchor protein; beige-like protein
UniProt Protein Name
Lipopolysaccharide-responsive and beige-like anchor protein
UniProt Synonym Protein Names
Beige-like protein
UniProt Gene Name
UniProt Synonym Gene Names
Bgl; Lba  [Similar Products]
UniProt Entry Name
LRBA_MOUSE
UniProt Comments for LRBA
LRBA: May be involved in coupling signal transduction and vesicle trafficking to enable polarized secretion and/or membrane deposition of immune effector molecules. Defects in LRBA are the cause of immunodeficiency, common variable, type 8, with autoimmunity (CVID8). An autosomal recessive immunologic disorder associated with defective B-cell differentiation and decreased or absent antibody production. Affected individuals have early-childhood onset of recurrent infections, particularly respiratory infections, and also develop variable autoimmune disorders, including idiopathic thrombocytopenic purpura, autoimmune hemolytic anemia, and inflammatory bowel disease. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: Golgi apparatus; extrinsic to membrane; membrane; cell; endoplasmic reticulum; lysosome; cytoplasmic membrane-bound vesicle; integral to membrane; plasma membrane; intracellular; trans-Golgi network

Molecular Function: protein kinase A binding; phospholipid binding

Biological Process: protein localization; endosome transport; signal transduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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