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LRP4 elisa kit :: Human Low Density Lipoprotein Receptor Related Protein 4 (LRP4) ELISA Kit

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Catalog # MBS2021747 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $270 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $665 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Low Density Lipoprotein Receptor Related Protein 4 (LRP4), ELISA Kit

Also Known As

Low Density Lipoprotein Receptor Related Protein 4 (LRP4) ELISA Kit

Product Synonym Names
MEGF7; LRP10; Multiple epidermal growth factor-like domains 7
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
1,905
OMIM
212780
3D Structure
ModBase 3D Structure for O75096
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Low Density Lipoprotein Receptor Related Protein 4 (LRP4).
No significant cross-reactivity or interference between Low Density Lipoprotein Receptor Related Protein 4 (LRP4) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.126ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Low Density Lipoprotein Receptor Related Protein 4 (LRP4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Low Density Lipoprotein Receptor Related Protein 4 (LRP4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LRP4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forLRP4purchase
MBS2021747 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Low Density Lipoprotein Receptor Related Protein 4 (LRP4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LRP4. The ELISA analytical biochemical technique of the MBS2021747 kit is based on LRP4 antibody-LRP4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LRP4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LRP4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LRP4 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Low Density Lipoprotein Receptor Related Protein 4 (LRP4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Low Density Lipoprotein Receptor Related Protein 4 (LRP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Low Density Lipoprotein Receptor Related Protein 4 (LRP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Low Density Lipoprotein Receptor Related Protein 4 (LRP4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for LRP4 elisa kit

Typical Testing Data/Standard Curve (for reference only) of LRP4 elisa kit
LRP4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2021747. Click to request current manual
NCBI/Uniprot data below describe general gene information for LRP4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
212,045 Da
NCBI Official Full Name
low-density lipoprotein receptor-related protein 4
NCBI Official Synonym Full Names
low density lipoprotein receptor-related protein 4
NCBI Official Symbol
NCBI Official Synonym Symbols
CLSS; CMS17; LRP-4; LRP10; MEGF7; SOST2
  [Similar Products]
NCBI Protein Information
low-density lipoprotein receptor-related protein 4
UniProt Protein Name
Low-density lipoprotein receptor-related protein 4
UniProt Synonym Protein Names
Multiple epidermal growth factor-like domains 7
UniProt Gene Name
UniProt Synonym Gene Names
KIAA0816; LRP10; MEGF7; LRP-4  [Similar Products]
UniProt Entry Name
LRP4_HUMAN
NCBI Summary for LRP4
This gene encodes a member of the low-density lipoprotein receptor-related protein family. The encoded protein may be a regulator of Wnt signaling. Mutations in this gene are associated with Cenani-Lenz syndrome. [provided by RefSeq, May 2010]
UniProt Comments for LRP4
LRP4: Mediates SOST-dependent inhibition of bone formation. Functions as a specific facilitator of SOST-mediated inhibition of Wnt signaling. Plays a key role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Directly binds AGRIN and recruits it to the MUSK signaling complex. Mediates the AGRIN- induced phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Alternatively, may be involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway. More generally, has been proposed to function as a cell surface endocytic receptor binding and internalizing extracellular ligands for degradation by lysosomes. Defects in LRP4 are the cause of Cenani-Lenz syndactyly syndrome (CLSS). It is a congenital malformation syndrome defined as complete and complex syndactyly of the hands combined with malformations of the forearm bones and similar manifestations in the lower limbs. Defects in LRP4 are the cause of sclerosteosis type 2 (SOST2). A sclerosing bone dysplasia characterized by a generalized hyperostosis and sclerosis leading to a markedly thickened skull, with mandible, ribs, clavicles and all long bones also being affected. Due to narrowing of the foramina of the cranial nerves, facial nerve palsy, hearing loss and atrophy of the optic nerves can occur. Sclerosteosis is clinically and radiologically very similar to van Buchem disease, mainly differentiated by hand malformations and a large stature in sclerosteosis patients. Belongs to the LDLR family.

Protein type: Cell surface; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: cell surface; cell soma; dendrite; postsynaptic density; integral to membrane; flotillin complex; neuromuscular junction

Molecular Function: protein binding; protein homodimerization activity; apolipoprotein binding; calcium ion binding; receptor tyrosine kinase binding

Biological Process: limb development; regulation of protein amino acid phosphorylation; extracellular matrix organization and biogenesis; Wnt receptor signaling pathway; dendrite morphogenesis; endocytosis; odontogenesis of dentine-containing teeth; hair follicle development; synaptic growth at neuromuscular junction; dorsal/ventral pattern formation; negative regulation of ossification; synapse organization and biogenesis; protein heterotetramerization; negative regulation of axonogenesis; embryonic digit morphogenesis; kidney development; proximal/distal pattern formation

Disease: Myasthenic Syndrome, Congenital, 17; Cenani-lenz Syndactyly Syndrome; Sclerosteosis 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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