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LTB4 elisa kit :: General LTB4 ELISA Kit

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Catalog # MBS2515771 (SPECIAL PROMOTIONAL PRICING for a limited time)
Unit / Price
  48-Strip-Wells  /  $310 +1 FREE 8GB USB
  96-Strip-Wells  /  $355 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,435 +1 FREE 8GB USB
  10x96-Strip-Wells  /  $2,805 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

LTB4, ELISA Kit

Popular Item
Also Known As

LTB4 (Leukotriene B4) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2515771 COA
Sequence Length
358
OMIM
605773
Species Reactivity
Specificity
This kit recognizes natural and some recombinant LTB4. No significant cross-reactivity or interference between LTB4 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Competitive
Detection Range
15.625-1000pg/mL
Sensitivity
Min: 9.375pg/mL; Max: 1000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LTB4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forLTB4purchase
MBS2515771 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the LTB4, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LTB4. The ELISA analytical biochemical technique of the MBS2515771 kit is based on LTB4 antibody-LTB4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LTB4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LTB4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LTB4 elisa kit
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of LTB4 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LTB4. During the reaction, LTB4 in the sample or standard competes with a fixed amount of LTB4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to LTB4. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the Substrate Reagent is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change can be measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of LTB4 in samples can be calculated by comparing the OD of the samples with the standard curve.

Typical Testing Data/Standard Curve (for reference only) of LTB4 elisa kit
LTB4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2515771. Click to request current manual
NCBI/Uniprot data below describe general gene information for LTB4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
37,942 Da
NCBI Official Full Name
LTB4 receptor JULF2
NCBI Official Synonym Full Names
leukotriene B4 receptor 2
NCBI Official Symbol
LTB4R2  [Similar Products]
NCBI Official Synonym Symbols
BLT2; NOP9; BLTR2; JULF2; KPG_004; LTB4-R2; LTB4-R 2
  [Similar Products]
NCBI Protein Information
leukotriene B4 receptor 2; LTB4 receptor JULF2; leukotriene B4 receptor BLT2; seven transmembrane receptor BLTR2
UniProt Protein Name
Leukotriene B4 receptor 2
UniProt Synonym Protein Names
LTB4 receptor JULF2; Leukotriene B4 receptor BLT2; Seven transmembrane receptor BLTR2
UniProt Gene Name
LTB4R2  [Similar Products]
UniProt Synonym Gene Names
BLT2R; BLTR2; LTB4-R 2; LTB4-R2  [Similar Products]
UniProt Entry Name
LT4R2_HUMAN
UniProt Comments for LTB4
LTB4R2: Low-affinity receptor for leukotrienes including leukotriene B4. Mediates chemotaxis of granulocytes and macrophages. The response is mediated via G-proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTB4 > 12-epi-LTB4 > LTB5 > LTB3. Belongs to the G-protein coupled receptor 1 family.

Protein type: Motility/polarity/chemotaxis; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: leukotriene B4 receptor activity; leukotriene receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of adenylate cyclase activity; chemotaxis; keratinocyte migration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withLTB4 elisa kit
 Reference Product  PubMed Publications
 LTB4R elisa kit  >24 publications with LTB4 and LTB4R
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