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LTC4S elisa kit :: Human Leukotriene C4 synthase, LTC4S ELISA Kit

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Catalog # MBS2602658
Unit / Price
  48-Strip-Wells  /  $260 +1 FREE 8GB USB
  96-Strip-Wells  /  $405 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Leukotriene C4 synthase, LTC4S, ELISA Kit

Popular Item
Also Known As

Human Leukotriene C4 synthase, LTC4S ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
246530
3D Structure
ModBase 3D Structure for Q16873
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant LTC4S concentration
Assay Type
Sandwich
Detection Range
20 ng/ml-0.312 ng/ml
Sensitivity
0.06 ng/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LTC4S elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forLTC4Spurchase
MBS2602658 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Leukotriene C4 synthase, LTC4S, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LTC4S. The ELISA analytical biochemical technique of the MBS2602658 kit is based on LTC4S antibody-LTC4S antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LTC4S antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LTC4S. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LTC4S elisa kit
Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human LTC4S monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of LTC4S elisa kit
LTC4S elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2602658. Click to request current manual
NCBI/Uniprot data below describe general gene information for LTC4S. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
16,567 Da
NCBI Official Full Name
leukotriene C4 synthase
NCBI Official Synonym Full Names
leukotriene C4 synthase
NCBI Official Symbol
LTC4S  [Similar Products]
NCBI Protein Information
leukotriene C4 synthase; LTC4 synthase
UniProt Protein Name
Leukotriene C4 synthase
UniProt Synonym Protein Names
Leukotriene-C(4) synthase
Protein Family
UniProt Gene Name
LTC4S  [Similar Products]
UniProt Synonym Gene Names
LTC4 synthase  [Similar Products]
UniProt Entry Name
LTC4S_HUMAN
NCBI Summary for LTC4S
The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family includes a number of human proteins, several of which are involved the production of leukotrienes. This gene encodes an enzyme that catalyzes the first step in the biosynthesis of cysteinyl leukotrienes, potent biological compounds derived from arachidonic acid. Leukotrienes have been implicated as mediators of anaphylaxis and inflammatory conditions such as human bronchial asthma. This protein localizes to the nuclear envelope and adjacent endoplasmic reticulum. [provided by RefSeq, Jul 2008]
UniProt Comments for LTC4S
LTC4S: Catalyzes the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4. Defects in LTC4S are the cause of leukotriene C4 synthase deficiency (LTC4 synthase deficiency). LTC4 synthase deficiency is a fatal neurometabolic developmental disorder. It is associated with muscular hypotonia, psychomotor retardation, failure to thrive, and microcephaly. Belongs to the MAPEG family.

Protein type: Endoplasmic reticulum; EC 4.4.1.20; Lyase; Membrane protein, integral; Membrane protein, multi-pass; Lipid Metabolism - arachidonic acid; Nuclear envelope

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: nuclear outer membrane; endoplasmic reticulum membrane; intracellular membrane-bound organelle; endoplasmic reticulum; integral to membrane; nuclear envelope

Molecular Function: protein binding; glutathione transferase activity; leukotriene-C4 synthase activity; enzyme activator activity; glutathione peroxidase activity; lipid binding

Biological Process: leukotriene biosynthetic process; positive regulation of catalytic activity; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process

Disease: Leukotriene C4 Synthase Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withLTC4S elisa kit
 Reference Product  PubMed Publications
 MGST1 elisa kit  >1 publications with LTC4S and MGST1
Organs/Tissues associated with LTC4S elisa kit
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