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LTF elisa kit :: Bovine lactotransferrin ELISA Kit

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Catalog # MBS704467
Unit / Price
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  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $590 +1 FREE 8GB USB
  96-Strip-Wells  /  $855 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,110 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,890 +7 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

lactotransferrin (LTF), ELISA Kit

Popular Item
Full Product Name

Bovine Lactoferrin, LTF/LF ELISA Kit

Product Synonym Names
Bovine Lactoferrin; LTF/LF ELISA kit; GIG12; HLF2; LF; growth-inhibiting protein 12; lactoferrin; neutrophil lactoferrin; talalactoferrin; lactotransferrin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS704467 Testing Data
3D Structure
ModBase 3D Structure for P24627
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Bovine LTF. No significant cross-reactivity or interference between Bovine LTF and analogues was observed.
Samples
Milk
Assay Type
Competitive
Detection Range
3.12 ug/ml-200 ug/ml
Sensitivity
3.12 ug/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Transport
Protein Biological Process 2
Anion transport
Protein Biological Process 3
Ion transport
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LTF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for LTFpurchase
MBS704467 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the lactotransferrin (LTF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LTF. The ELISA analytical biochemical technique of the MBS704467 kit is based on LTF antibody-LTF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LTF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LTF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LTF elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to LTF/LF has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated LTF/LF and incubated. A competitive inhibition reaction is launched between LTF/LF (Standards or samples) and HRP-conjugated LTF/LF with the pre-coated antibody specific for LTF/LF. The more amount of LTF/LF in samples, the less antibody bound by HRP-conjugated LTF/LF. Then the substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of LTF/LF in the sample. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of LTF elisa kit
LTF elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS704467. Click to request current manual
NCBI/Uniprot data below describe general gene information for LTF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
78,056 Da
NCBI Official Full Name
lactotransferrin
NCBI Official Synonym Full Names
lactotransferrin
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
lactotransferrin; lactoferrin
UniProt Protein Name
Lactotransferrin
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Lactoferrin; Lfcin-B  [Similar Products]
UniProt Entry Name
TRFL_BOVIN
UniProt Comments for LTF
Function: Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate.5 PublicationsManual assertion based on experiment in:Ref.8

Catalytic activity: Preferential at -Arg-Ser-Arg-Arg-|- and -Arg-Arg-Ser-Arg-|-, and of Z-Phe-Arg-|-aminomethylcoumarin.

Enzyme regulation: Inhibited by PMSF and Pefabloc.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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