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LTF elisa kit :: Ovotransferrin ELISA Kit

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Catalog # MBS564009
Unit / Price
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  1 Plate  /  $480 +1 FREE 8GB USB
LTF elisa kit
Product Name

Ovotransferrin (LTF), ELISA Kit

Popular Item
Full Product Name

Chicken Ovotransferrin (Conalbumin) ELISA Kit

Product Synonym Names
Chicken Ovotransferrin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS564009 COA
Chromosome Location
Chromosome: 9; NC_006096.2 (5631644..5643602, complement)
3D Structure
ModBase 3D Structure for P02789
Host
Chicken
Samples
Biological samples of Chickens
Assay Type
Sandwich
Preparation and Storage
Store at 4 degree C.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LTF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for LTFpurchase
MBS564009 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Chicken Ovotransferrin (LTF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LTF. The ELISA analytical biochemical technique of the MBS564009 kit is based on LTF antibody-LTF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LTF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LTF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). This product is a highly sensitive ELISA based assay kit for detecting and quantifying chicken ovotransferrin (conalbumin). In this indirect ovotransferrin conalbumin ELISA assay, known amounts of anti-chicken ovotransferrin antibodies are adsorbed to each plate well. A sample containing chicken ovotransferrin is added to each well and binds to capture antibodies. Unbound antigen is washed away. Subsequently, a detection antibody solution containing anti-ovotransferrin antibodies conjugated with horseradish peroxidase is added, forming a complex with the previously bound chicken ovotransferrin and anti-ovotransferrin capture antibodies. Another wash removes unbound detection antibodies, and a chromogenic substrate is added. The amount of bound enzyme varies directly with the concentration of protein in the original test sample, thus the absorbance at 450nm is inversely proportional with the concentration of conalbumin added to each well. The concentration of ovotransferrin in the test sample can be derived from a calibration curve of known standards.
Related Product Information for
LTF elisa kit
Intended Uses: The total Chicken Ovotransferrin (TX) test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Ovotransferrin in biological samples of Chickens.

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the TX present in samples reacts with the anti-TX antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-TX antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound TX. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of TX in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of TX in the test sample. The quantity of TX in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Product Categories/Family for LTF elisa kit
Sample Manual Insert of MBS564009. Click to request current manual
NCBI/Uniprot data below describe general gene information for LTF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
77,777 Da[Similar Products]
NCBI Official Full Name
ovotransferrin
NCBI Official Symbol
NCBI Official Synonym Symbols
TF; TFEW; conalbumin
  [Similar Products]
NCBI Protein Information
ovotransferrin; serum transferrin; allergen Gal d III; ovotransferrin BB type; ovotransferrin BC type; ovotransferrin CC type
UniProt Protein Name
Ovotransferrin
UniProt Synonym Protein Names
Allergen Gal d III; Conalbumin; Serum transferrin
Protein Family
UniProt Entry Name
TRFE_CHICK
UniProt Comments for LTF
Function: Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.Transferrin has a bacteriostatic function. Its concentration in avian egg is the highest concentration of any transferrin in vivo. Transferrin in liver is regulated by the iron levels but not in the oviduct. These two forms of transferrin (liver and oviduct) differ only by their carbohydrate composition.

Subunit structure: Monomer.

Subcellular location: Secreted.

Allergenic properties: Causes an allergic reaction in human.

Sequence similarities: Belongs to the transferrin family.Contains 2 transferrin-like domains.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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