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LXRa elisa kit :: Mouse Liver X Receptor Alpha (LXRa) ELISA Kit

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Catalog # MBS2020587 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $270 +1 FREE 8GB USB
  48-Strip-Wells  /  $505 +1 FREE 8GB USB
  96-Strip-Wells  /  $685 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,770 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,025 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Liver X Receptor Alpha (LXRa), ELISA Kit

Full Product Name

Liver X Receptor Alpha (LXRa) ELISA Kit

Product Synonym Names
NR1-H3; LXR-A; LXRA; RLD-1; Nuclear Receptor Subfamily 1,Group H,Member 3; Oxysterols receptor LXR-alpha
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
602423
3D Structure
ModBase 3D Structure for Q13133
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Liver X Receptor Alpha (LXRa).
No significant cross-reactivity or interference between Liver X Receptor Alpha (LXRa) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates and Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.103ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Liver X Receptor Alpha (LXRa) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Liver X Receptor Alpha (LXRa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LXRa elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forLXRapurchase
MBS2020587 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Liver X Receptor Alpha (LXRa) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LXRa. The ELISA analytical biochemical technique of the MBS2020587 kit is based on LXRa antibody-LXRa antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LXRa antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LXRa. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LXRa elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Liver X Receptor Alpha (LXRa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Liver X Receptor Alpha (LXRa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Liver X Receptor Alpha (LXRa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Liver X Receptor Alpha (LXRa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of LXRa elisa kit
LXRa elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2020587. Click to request current manual
NCBI/Uniprot data below describe general gene information for LXRa. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
45,692 Da
NCBI Official Full Name
liver X receptor alpha
NCBI Official Synonym Full Names
nuclear receptor subfamily 1, group H, member 3
NCBI Official Symbol
NR1H3  [Similar Products]
NCBI Official Synonym Symbols
LXRA; LXR-a; RLD-1
  [Similar Products]
NCBI Protein Information
oxysterols receptor LXR-alpha
UniProt Protein Name
Oxysterols receptor LXR-alpha
UniProt Synonym Protein Names
Liver X receptor alpha; Nuclear receptor subfamily 1 group H member 3
UniProt Gene Name
NR1H3  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
NR1H3_HUMAN
NCBI Summary for LXRa
The protein encoded by this gene belongs to the NR1 subfamily of the nuclear receptor superfamily. The NR1 family members are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. This protein is highly expressed in visceral organs, including liver, kidney and intestine. It forms a heterodimer with retinoid X receptor (RXR), and regulates expression of target genes containing retinoid response elements. Studies in mice lacking this gene suggest that it may play an important role in the regulation of cholesterol homeostasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
UniProt Comments for LXRa
LXR-alpha: Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand- binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half- sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Belongs to the nuclear hormone receptor family. NR1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: nucleoplasm; nuclear chromatin; nucleus; receptor complex

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; sterol response element binding; DNA binding; zinc ion binding; sequence-specific DNA binding; transcription coactivator activity; cholesterol binding; steroid hormone receptor activity

Biological Process: negative regulation of lipid transport; transcription initiation from RNA polymerase II promoter; positive regulation of fatty acid biosynthetic process; intracellular receptor-mediated signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; positive regulation of toll-like receptor 4 signaling pathway; lipid homeostasis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of pinocytosis; positive regulation of cholesterol transport; regulation of circadian rhythm; cholesterol homeostasis; negative regulation of inflammatory response; positive regulation of lipoprotein lipase activity; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; response to progesterone stimulus; sterol homeostasis; negative regulation of macrophage activation; apoptotic cell clearance
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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