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HepG2 Cell Lysate, Vanadate Treated Lysate

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Catalog # MBS657197
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  1 Each  /  $425 +1 FREE 8GB USB
HepG2 Cell Lysate, Vanadate Treated lysate
Product Name

HepG2 Cell Lysate, Vanadate Treated, Lysate

Popular Item
Full Product Name

HepG2 Cell Lysate, Vanadate Treated

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Host
Pervanadate treated HepG2 cells.
Specificity
Pervandate treated HepG2 cells. Serum starved cells were treated for 15 min with pervandate. 20ul is ~80,000 cells.
Purity/Purification
Lysate
Form/Format
Supplied as a liquid.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of HepG2 Cell Lysate, Vanadate Treated lysate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for HepG2 Cell Lysate, Vanadate Treated lysate
Western Blot (WB)
Suitable for use as a positive control in Western Blot applications. Not recommended for use in Immunoprecipitation.

Recommended Dilutions: Add 100ul SDS-PAGE sample buffer and incubate at 90 Degree C for 5 minutes. Aliquot unused lysate and store at -20 Deg. C. Western Blot: 20ul/lane (mini gel) for HRP/ECL detection. Optimal dilutions to be determined by the researcher.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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