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M-AChRM1 elisa kit :: Human M-AChR M1 ELISA Kit

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Catalog # MBS2501151
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

M-AChR M1 (M-AChRM1), ELISA Kit

Popular Item
Full Product Name

Human M-AChR M1 (Muscarinic Acetylcholine Receptor M1) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for P12657
Species Reactivity
Specificity
This kit recognizes natural and recombinant Human M-AChRM1. No significant cross-reactivity or interference between Human M-AChRM1 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of M-AChRM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forM-AChRM1purchase
MBS2501151 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the M-AChR M1 (M-AChRM1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing M-AChRM1. The ELISA analytical biochemical technique of the MBS2501151 kit is based on M-AChRM1 antibody-M-AChRM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect M-AChRM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, M-AChRM1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
M-AChRM1 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human M-AChRM1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to M-AChRM1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for M-AChRM1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain M-AChRM1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of M-AChRM1. You can calculate the concentration of M-AChRM1 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of M-AChRM1 elisa kit
M-AChRM1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2501151. Click to request current manual
NCBI/Uniprot data below describe general gene information for M-AChRM1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
51,379 Da
NCBI Official Full Name
muscarinic acetylcholine receptor M1
NCBI Official Synonym Full Names
cholinergic receptor, muscarinic 1, CNS
NCBI Official Symbol
Chrm1  [Similar Products]
NCBI Official Synonym Symbols
M1; M1R; Chrm-1
  [Similar Products]
NCBI Protein Information
muscarinic acetylcholine receptor M1; AChR M1; m1 muscarinic acetylcholine receptor protein
UniProt Protein Name
Muscarinic acetylcholine receptor M1
UniProt Gene Name
Chrm1  [Similar Products]
UniProt Synonym Gene Names
Chrm-1  [Similar Products]
UniProt Entry Name
ACM1_MOUSE
UniProt Comments for M-AChRM1
mAChR m1: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM1 sub-subfamily.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral

Cellular Component: asymmetric synapse; postsynaptic membrane; membrane; integral to plasma membrane; dendrite; postsynaptic density; integral to membrane; plasma membrane; synapse; nerve terminal; cell junction

Molecular Function: G-protein coupled receptor activity; signal transducer activity; drug binding; G-protein coupled acetylcholine receptor activity

Biological Process: regulation of locomotion; G-protein coupled receptor protein signaling pathway; acetylcholine receptor signaling, muscarinic pathway; neuromuscular synaptic transmission; positive regulation of ion transport; cognition; signal transduction; saliva secretion
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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