• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-MADD antibody :: MADD Antibody

Scan QR to view Datasheet
Catalog # MBS851663
Unit / Price
  0.1 mg  /  $215 +1 FREE 8GB USB
Product Name

MADD, Antibody

Also Known As

MADD Antibody

Product Synonym Names
MAP kinase-activating death domain protein; Differentially expressed in normal and neoplastic cells; Insulinoma glucagonoma clone 20; Rab3 GDP/GTP exchange factor; MADD; DENN; IG20; KIAA0358
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
3D Structure
ModBase 3D Structure for Q8WXG6
Species Reactivity
Human, Mouse, Rat
MADD antibody detects endogenous levels of total MADD protein.
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
The antiserum was produced against synthesized peptide derived from internal of human MADD.
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-MADD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-MADD antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-MADD antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:1000

Testing Data of anti-MADD antibody
anti-MADD antibody Testing Data image
Immunohistochemistry (IHC) of anti-MADD antibody
Immunohistochemistry analysis of paraffin-embedded human brain tissue using MADD antibody. Western blot analysis of extracts from COLO cells, HeLa cells and Jurkat cells, using MADD antibody.
anti-MADD antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-MADD antibody
Peptide-+ Immunofluorescence analysis of A549 cells, using MADD antibody.
anti-MADD antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for MADD. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
171,006 Da
NCBI Official Full Name
MAP kinase-activating death domain protein isoform i
NCBI Official Synonym Full Names
MAP kinase activating death domain
NCBI Official Symbol
NCBI Official Synonym Symbols
  [Similar Products]
NCBI Protein Information
MAP kinase-activating death domain protein
UniProt Protein Name
MAP kinase-activating death domain protein
UniProt Synonym Protein Names
Differentially expressed in normal and neoplastic cells; Insulinoma glucagonoma clone 20; Rab3 GDP/GTP exchange factor
UniProt Gene Name
NCBI Summary for MADD
Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. The apoptotic signal is transduced inside these cells by cytoplasmic adaptor proteins. The protein encoded by this gene is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for MADD
Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows decreased. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis); isoform 1 is susceptible to inducing apoptosis, isoform 5 is resistant and isoform 3 and isoform 4 have no effect.
Product References and Citations for anti-MADD antibody
Chow V.T.K., DNA Seq. 6:263-273(1996). Schievella A.R., J. Biol. Chem. 272:12069-12075(1997). Chow V.T.K., Genome 41:543-552(1998).

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

Contact Us

Please fill out the form below and our representative will get back to you shortly.