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MAGED4B elisa kit :: Human melanoma antigen family D, 4B ELISA Kit

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Catalog # MBS701702
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

melanoma antigen family D, 4B (MAGED4B), ELISA Kit

Also Known As

Human Melanoma-associated antigen D4, MAGED4/MAGED4B ELISA Kit

Product Synonym Names
Human Melanoma-associated antigen D4 (MAGED4/MAGED4B) ELISA Kit; melanoma antigen family D; 4B
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS701702 Testing Data
Sequence Length
739
OMIM
gene 300765
3D Structure
ModBase 3D Structure for Q96JG8
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human MAGED4B. No significant cross-reactivity or interference between Human MAGED4B and analogues was observed.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MAGED4B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forMAGED4Bpurchase
MBS701702 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the melanoma antigen family D, 4B (MAGED4B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MAGED4B. The ELISA analytical biochemical technique of the MBS701702 kit is based on MAGED4B antibody-MAGED4B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MAGED4B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MAGED4B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MAGED4B elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAGED4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MAGED4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain MAGED4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MAGED4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of MAGED4B elisa kit
MAGED4B elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS701702. Click to request current manual
NCBI/Uniprot data below describe general gene information for MAGED4B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
81,378 Da
NCBI Official Full Name
melanoma-associated antigen D4 isoform 2
NCBI Official Synonym Full Names
melanoma antigen family D, 4
NCBI Official Symbol
MAGED4  [Similar Products]
NCBI Official Synonym Symbols
MAGE1; MAGEE1; MAGE-E1
  [Similar Products]
NCBI Protein Information
melanoma-associated antigen D4; MAGE-D4 antigen; MAGE-E1 antigen
UniProt Protein Name
Melanoma-associated antigen D4
UniProt Synonym Protein Names
MAGE-D4 antigen; MAGE-E1 antigen
UniProt Gene Name
MAGED4  [Similar Products]
UniProt Synonym Gene Names
KIAA1859; MAGED4A; MAGEE1
MAGED4B  [Similar Products]
UniProt Entry Name
MAGD4_HUMAN
UniProt Comments for MAGED4B
Function: May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.1 PublicationManual assertion based on experiment in:Ref.13
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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