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MAGOH recombinant protein

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Catalog # MBS203529
Unit / Price
  0.1 mg  /  $285 +1 FREE 8GB USB
  0.5 mg  /  $640 +1 FREE 8GB USB
SDS-Page
Product Name

MAGOH, Recombinant Protein

Also Known As

MAGOH, 1-146aa, Human, His tag, E Coli

Product Synonym Names
Protein mago nashi homolog; MAGOH1; MAGOHA; Protein mago nashi homolog Mago nashi homolog proliferation associated (Drosophila); Mago nashi protein homolog.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
146
Sequence
MESDFYLRYY VGHKGKFGHE FLEFEFRPDG KLRYANNSNY KNDVMIRKEA YVHKSVMEEL KRIIDDSEIT KEDDALWPPP DRVGRQELEI VIGDEHISFT TSKIGSLIDV NQSKDPEGLR VFYYLVQDLK CLVFSLIGLH FKIKPILEHH HHHH
OMIM
AF035940 mRNA
3D Structure
ModBase 3D Structure for P61326
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 2mM DTT, 0.1M NaCl, 20% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of MAGOH recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MAGOH recombinant protein
MAGOH, the human homolog of Drosophila mago nashi, is required for embryo development. MAGOH is ubiquitously expressed in adult tissues. In mammals, mRNA expression is not limited to the germ plasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. Recombinant human MAGOH protein, fused to His-tag at C-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Applications Tested/Suitable for MAGOH recombinant protein
SDS-PAGE

NCBI/Uniprot data below describe general gene information for MAGOH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
18.2 kDa (154aa), confirmed by MALDI-TOF
NCBI Official Full Name
protein mago nashi homolog
NCBI Official Synonym Full Names
mago homolog, exon junction complex core component
NCBI Official Symbol
MAGOH  [Similar Products]
NCBI Official Synonym Symbols
MAGOH1; MAGOHA
  [Similar Products]
NCBI Protein Information
protein mago nashi homolog
UniProt Protein Name
Protein mago nashi homolog
Protein Family
UniProt Gene Name
MAGOH  [Similar Products]
UniProt Synonym Gene Names
MAGOHA  [Similar Products]
NCBI Summary for MAGOH
Drosophila that have mutations in their mago nashi (grandchildless) gene produce progeny with defects in germplasm assembly and germline development. This gene encodes the mammalian mago nashi homolog. In mammals, mRNA expression is not limited to the germ plasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. [provided by RefSeq, Jul 2008]
UniProt Comments for MAGOH
Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly.
Product References and Citations for MAGOH recombinant protein
Gehring NH., et al. (2009) Cell. 137 (3):536-48. ; Chamieh H., et al. (2008) Nat Struct Mol Biol. 15 (1):85-93.;

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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