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MAOB elisa kit :: Mouse Monoamine Oxidase B (MAOB) ELISA Kit

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Catalog # MBS2024919 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Monoamine Oxidase B (MAOB), ELISA Kit

Full Product Name

Monoamine Oxidase B (MAOB) ELISA Kit

Product Synonym Names
MAO-B; Amine oxidase [flavin-containing] B
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
309860
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Monoamine Oxidase B (MAOB).
No significant cross-reactivity or interference between Monoamine Oxidase B (MAOB) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates and Other Biological Fluids
Detection Range
31.25-2000pg/mL
Sensitivity
< 12.4pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Monoamine Oxidase B (MAOB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Monoamine Oxidase B (MAOB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MAOB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for MAOBpurchase
MBS2024919 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Monoamine Oxidase B (MAOB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MAOB. The ELISA analytical biochemical technique of the MBS2024919 kit is based on MAOB antibody-MAOB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MAOB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MAOB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MAOB elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monoamine Oxidase B (MAOB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Monoamine Oxidase B (MAOB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Monoamine Oxidase B (MAOB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Monoamine Oxidase B (MAOB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for MAOB elisa kit

Typical Testing Data/Standard Curve (for reference only) of MAOB elisa kit
MAOB elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2024919. Click to request current manual
NCBI/Uniprot data below describe general gene information for MAOB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
46,539 Da
NCBI Official Full Name
monoamine oxidase B, partial
NCBI Official Synonym Full Names
monoamine oxidase B
NCBI Official Symbol
NCBI Protein Information
amine oxidase [flavin-containing] B
UniProt Protein Name
Amine oxidase [flavin-containing] B
UniProt Synonym Protein Names
Monoamine oxidase type B; MAO-B
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
MAO-B  [Similar Products]
UniProt Entry Name
AOFB_HUMAN
NCBI Summary for MAOB
The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous sysytem and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine. [provided by RefSeq, Jul 2008]
UniProt Comments for MAOB
MAOB: Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine. Belongs to the flavin monoamine oxidase family.

Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - arginine and proline; Membrane protein, integral; Mitochondrial; Oxidoreductase; Amino Acid Metabolism - histidine; EC 1.4.3.4; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - glycine, serine and threonine

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: mitochondrial envelope; mitochondrial outer membrane; mitochondrion; mitochondrial inner membrane; integral to membrane

Molecular Function: amine oxidase activity; protein homodimerization activity; electron carrier activity; FAD binding

Biological Process: response to drug; negative regulation of serotonin secretion; positive regulation of dopamine metabolic process; response to selenium ion; response to ethanol; substantia nigra development; dopamine catabolic process; response to toxin; xenobiotic metabolic process; response to lipopolysaccharide; hydrogen peroxide biosynthetic process; response to corticosterone stimulus; response to aluminum ion
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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