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anti-MAPKAPK2 antibody :: Rabbit MAPKAPK2 (Phospho-Ser272) Antibody

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Catalog # MBS004662
Unit / Price
  0.05 mL  /  $270 +1 FREE 8GB USB
  0.1 mL  /  $445 +1 FREE 8GB USB
Product Name

MAPKAPK2 (Phospho-Ser272), Antibody

Popular Item
Also Known As

Rabbit MAPKAPK2 (Phospho-Ser272) Antibody

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
370
Chromosome Location
Chromosome: 1; NC_000001.10 (206858365..206907630). Location: 1q32
OMIM
602006
3D Structure
ModBase 3D Structure for P49137
Isotype
IgG
Host
Rabbit
Species Reactivity
Human (Identities = 100%, Positives = 100%);
Mouse (Identities = 100%, Positives = 100%);
Rat (Identities = 100%, Positives = 100%)
Specificity
MAPKAPK2 (Phospho-Ser272) antibody detects endogenous levels of MAPKAPK2 only when phosphorylated at serine 272.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
All Sites
Human: Ser272; Mouse: Ser258; Rat: Ser258
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human MAPKAPK2 around the phosphorylation site of serine 272 (A-I-SP-P-G).
Preparation and Storage
Store at -20 Degree C/1 year
Other Notes
Small volumes of anti-MAPKAPK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-MAPKAPK2 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-MAPKAPK2 antibody
WB: 1:500~1:3000
IHC: 1:50~1:100
ELISA: 1:1000

Immunohistochemistry of anti-MAPKAPK2 antibody
Immunohistochemistry analysis of paraffin-embedded human brain tissue using MAPKAPK2 (Phospho-Ser272) antibody.
anti-MAPKAPK2 antibody Immunohistochemistry (IHC) image
Western Blot of anti-MAPKAPK2 antibody
Western blot analysis of extracts from RAW264.7 cells, treated with UV (15mins), using MAPKAPK2 (Phospho-Ser272) antibody.
anti-MAPKAPK2 antibody  Western Blot (WB) image
NCBI/Uniprot data below describe general gene information for MAPKAPK2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
45,568 Da
NCBI Official Full Name
MAP kinase-activated protein kinase 2 isoform 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase-activated protein kinase 2
NCBI Official Symbol
MAPKAPK2  [Similar Products]
NCBI Official Synonym Symbols
MK2; MK-2; MAPKAP-K2
  [Similar Products]
NCBI Protein Information
MAP kinase-activated protein kinase 2; MAPKAP kinase 2; MAPK-activated protein kinase 2
UniProt Protein Name
MAP kinase-activated protein kinase 2
UniProt Gene Name
MAPKAPK2  [Similar Products]
UniProt Synonym Gene Names
MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2  [Similar Products]
UniProt Entry Name
MAPK2_HUMAN
NCBI Summary for MAPKAPK2
This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for MAPKAPK2
MAPKAPK2: a member of the MAPKAPK family of protein kinases. It is phosphorylated and activated by p38 in response to cytokines, stress and chemotactic factors.MAPKAPK-2 is apparently a direct target of p38 MAPK. HSP27 is one of its in vivo substrates. Two transcript variants encoding two different isoforms have been described.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Protein kinase, CAMK; CAMK group; MAPKAPK family; MAPKAPK subfamily

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: nucleoplasm; centrosome; cytoplasm; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; signal transducer activity; protein binding; ATP binding; protein kinase activity

Biological Process: nerve growth factor receptor signaling pathway; activation of MAPK activity; stress-activated MAPK cascade; response to lipopolysaccharide; leukotriene metabolic process; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of interleukin-6 production; arachidonic acid metabolic process; response to stress; inflammatory response; toll-like receptor 4 signaling pathway; inner ear development; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; peptidyl-serine phosphorylation; response to cytokine stimulus; Ras protein signal transduction; toll-like receptor signaling pathway; innate immune response; regulation of tumor necrosis factor production; gene expression; toll-like receptor 9 signaling pathway; vascular endothelial growth factor receptor signaling pathway; G2/M transition DNA damage checkpoint; response to DNA damage stimulus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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