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MASP2 elisa kit :: Human mannan-binding lectin serine peptidase 2 ELISA Kit

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Catalog # MBS941977
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $550 +1 FREE 8GB USB
  96-Strip-Wells  /  $795 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

mannan-binding lectin serine peptidase 2 (MASP2), ELISA Kit

Popular Item
Also Known As

Human mannan-binding lectin serine peptidase 2, MASP2 ELISA Kit

Product Synonym Names
Human mannan-binding lectin serine peptidase 2 (MASP2) ELISA kit; MAP19; MASP-2; sMAP; MBL-associated plasma protein of 19 kD; MBL-associated serine protease 2; mannan-binding lectin serine protease 2; small MBL-associated protein; mannan-binding lectin serine peptidase 2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS941977 Testing Data
Sequence Length
686
OMIM
gene 613791
3D Structure
ModBase 3D Structure for O00187
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human MASP2. No significant cross-reactivity or interference between human MASP2 and analogues was observed.
Samples
Serum, plasma, cell culture supernates and cerebrospinal fluid (CSF)
Assay Type
Sandwich
Detection Range
23.44 pg/ml -1500 pg/ml.
Sensitivity
The minimum detectable dose of human MASP2 is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MASP2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forMASP2purchase
MBS941977 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the mannan-binding lectin serine peptidase 2 (MASP2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MASP2. The ELISA analytical biochemical technique of the MBS941977 kit is based on MASP2 antibody-MASP2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MASP2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MASP2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MASP2 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MASP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MASP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MASP2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MASP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of MASP2 elisa kit
MASP2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS941977. Click to request current manual
NCBI/Uniprot data below describe general gene information for MASP2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
75,702 Da
NCBI Official Full Name
mannan-binding lectin serine protease 2 isoform 1 preproprotein
NCBI Official Synonym Full Names
mannan-binding lectin serine peptidase 2
NCBI Official Symbol
MASP2  [Similar Products]
NCBI Official Synonym Symbols
sMAP; MAP19; MASP-2; MASP1P1
  [Similar Products]
NCBI Protein Information
mannan-binding lectin serine protease 2; small MBL-associated protein; MBL-associated serine protease 2; MBL-associated plasma protein of 19 kD; mannan-binding lectin serine protease 1 pseudogene 1; mannose-binding protein-associated serine protease 2; mannan-binding lectin serine peptidase 1 pseudogene 1
UniProt Protein Name
Mannan-binding lectin serine protease 2
UniProt Synonym Protein Names
MBL-associated serine protease 2; Mannose-binding protein-associated serine protease 2; MASP-2
UniProt Gene Name
MASP2  [Similar Products]
UniProt Synonym Gene Names
MASP-2  [Similar Products]
UniProt Entry Name
MASP2_HUMAN
NCBI Summary for MASP2
The Ra-reactive factor (RARF) is a complement-dependent bactericidal factor that binds to the Ra and R2 polysaccharides expressed by certain enterobacteria. Alternate splicing of this gene results in two transcript variants encoding two RARF components that are involved in the mannan-binding lectin pathway of complement activation. The longer isoform is cleaved into two chains which form a heterodimer linked by a disulfide bond. The encoded proteins are members of the trypsin family of peptidases. [provided by RefSeq, Jul 2008]
UniProt Comments for MASP2
MASP2: Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase. Defects in MASP2 are the cause of MASP2 deficiency (MASPD). MASPD is a disorder that results in autoimmune manifestations, recurrent severe infections, and chronic inflammatory disease. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.21.104; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.3-p36.2

Cellular Component: extracellular region

Molecular Function: peptidase activity; protein binding; serine-type endopeptidase activity; complement component C4b binding; calcium ion binding; calcium-dependent protein binding

Biological Process: innate immune response; complement activation, lectin pathway; proteolysis; complement activation, classical pathway; complement activation

Disease: Masp2 Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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