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MBOAT4 elisa kit :: Human membrane bound O-acyltransferase domain containing 4 ELISA Kit

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Catalog # MBS929750
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Typical Testing Data/Standard Curve (for reference only)
Product Name

membrane bound O-acyltransferase domain containing 4 (MBOAT4), ELISA Kit

Popular Item
Also Known As

Human Ghrelin O-acyltransferase, MBOAT4 ELISA Kit

Product Synonym Names
Human Ghrelin O-acyltransferase (MBOAT4) ELISA kit; FKSG89; GOAT; OACT4; O-acyltransferase (membrane bound) domain containing 4; ghrelin O-acyltransferase; membrane bound O-acyltransferase domain containing 4
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS929750 Testing Data
Sequence Length
435
OMIM
611940
3D Structure
ModBase 3D Structure for Q96T53
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human MBOAT4. No significant cross-reactivity or interference between human MBOAT4 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
15.6 pg/ml -1000 pg/ml.
Sensitivity
The minimum detectable dose of human MBOAT4 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MBOAT4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forMBOAT4purchase
MBS929750 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the membrane bound O-acyltransferase domain containing 4 (MBOAT4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MBOAT4. The ELISA analytical biochemical technique of the MBS929750 kit is based on MBOAT4 antibody-MBOAT4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MBOAT4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MBOAT4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MBOAT4 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MBOAT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MBOAT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MBOAT4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBOAT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of MBOAT4 elisa kit
MBOAT4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS929750. Click to request current manual
NCBI/Uniprot data below describe general gene information for MBOAT4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
49,716 Da
NCBI Official Full Name
ghrelin O-acyltransferase
NCBI Official Synonym Full Names
membrane bound O-acyltransferase domain containing 4
NCBI Official Symbol
MBOAT4  [Similar Products]
NCBI Official Synonym Symbols
GOAT; OACT4; FKSG89
  [Similar Products]
NCBI Protein Information
ghrelin O-acyltransferase; O-acyltransferase domain-containing protein 4; O-acyltransferase (membrane bound) domain containing 4; membrane-bound O-acyltransferase domain-containing protein 4
UniProt Protein Name
Ghrelin O-acyltransferase
UniProt Synonym Protein Names
Membrane-bound O-acyltransferase domain-containing protein 4; O-acyltransferase domain-containing protein 4
Protein Family
UniProt Gene Name
MBOAT4  [Similar Products]
UniProt Synonym Gene Names
GOAT; OACT4; FKSG89; O-acyltransferase domain-containing protein 4  [Similar Products]
UniProt Entry Name
MBOA4_HUMAN
UniProt Comments for MBOAT4
MBOAT4: Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid. Belongs to the membrane-bound acyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Endoplasmic reticulum; Membrane protein, integral; Membrane protein, multi-pass; EC 2.3.1.-

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane

Molecular Function: serine O-acyltransferase activity

Biological Process: cellular protein metabolic process; peptidyl-serine octanoylation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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