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MCL1 elisa kit :: Human Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) ELISA Kit

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Catalog # MBS2019617 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
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  96-Strip-Wells  /  $665 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1), ELISA Kit

Popular Item
Full Product Name

Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) ELISA Kit

Product Synonym Names
EAT; MCL1L; MCL1S; BCL2L3; TM; Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; mcl1/EAT
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
159552
3D Structure
ModBase 3D Structure for Q07820
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1).
No significant cross-reactivity or interference between Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.127ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MCL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for MCL1purchase
MBS2019617 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MCL1. The ELISA analytical biochemical technique of the MBS2019617 kit is based on MCL1 antibody-MCL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MCL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MCL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MCL1 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for MCL1 elisa kit

Typical Testing Data/Standard Curve (for reference only) of MCL1 elisa kit
MCL1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2019617. Click to request current manual
NCBI/Uniprot data below describe general gene information for MCL1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
28,662 Da
NCBI Official Full Name
myeloid cell leukemia sequence 1
NCBI Official Synonym Full Names
myeloid cell leukemia 1
NCBI Official Symbol
NCBI Official Synonym Symbols
TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
  [Similar Products]
NCBI Protein Information
induced myeloid leukemia cell differentiation protein Mcl-1
UniProt Protein Name
Induced myeloid leukemia cell differentiation protein Mcl-1
UniProt Synonym Protein Names
Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT/mcl1; mcl1/EAT
UniProt Gene Name
UniProt Synonym Gene Names
BCL2L3; Bcl2-L-3  [Similar Products]
UniProt Entry Name
MCL1_HUMAN
NCBI Summary for MCL1
This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]
UniProt Comments for MCL1
MCL1: a myeloid cell leukemia protein of the Bcl-2 family of proteins. Two alternatively spliced transcripts encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.

Protein type: Apoptosis; Channel, misc.; Membrane protein, integral; Mitochondrial; Inhibitor

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; membrane; mitochondrial matrix; cytoplasm; integral to membrane; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; protein channel activity; BH3 domain binding

Biological Process: apoptotic mitochondrial changes; DNA damage response, signal transduction resulting in induction of apoptosis; response to cytokine stimulus; multicellular organismal development; cell fate determination; cellular homeostasis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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