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mCNTF recombinant protein :: Ciliary Neurotrophic Factor Recombinant Protein

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Catalog # MBS144453
Unit / Price
  0.005 mg  /  $140 +1 FREE 8GB USB
  0.025 mg  /  $205 +1 FREE 8GB USB
  1 mg  /  $2,665 +3 FREE 8GB USB
mCNTF recombinant protein
Product Name

Ciliary Neurotrophic Factor (mCNTF), Recombinant Protein

Popular Item
Also Known As

Recombinant Mouse Ciliary Neurotrophic Factor

Product Synonym Names
CNTF Mouse; Ciliary-Neurotrophic Factor Mouse Recombinant; HCNTF; CNTF; Ciliary Neurotrophic Factor
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
198
Sequence
MAFAEQSPLT LHRRDLCSRS IWLARKIRSD LTALMESYVK HQGLNKNISL DSVDGVPVAS TDRWSEMTEA ERLQENLQAY RTFQGMLTKL LEDQRVHFTP TEGDFHQAIH TLTLQVSAFA YQLEELMALL EQKVPEKEAD GMPVTIGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRVIS SHHMGISAHE SHYGAKQM
3D Structure
ModBase 3D Structure for P51642
Host
E Coli
Purity/Purification
Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Form/Format
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the lyophilized CNTF in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of TF-1 cells is less than 35ng/ml, corresponding to a Specific Activity of 3.0x104 IU/mg.
Preparation and Storage
Lyophilized Ciliary Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CNTF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.
Other Notes
Small volumes of mCNTF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
mCNTF recombinant protein
Description: Ciliary Neurotrophic Factor Recombinant Mouse produced in E Coli is a single, non-glycosylated polypeptide chain containing 198 amino acids and having a molecular mass of 22.6kDa. The CNTF is purified by proprietary chromatographic techniques.

Introduction: CNTF is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. In addition to the predominant monocistronic transcript originating from this locus, the gene is also co-transcribed with the upstream ZFP91 gene. Co-transcription from the two loci results in a transcript that contains a complete coding region for the zinc finger protein but lacks a complete coding region for ciliary neurotrophic factor.CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
NCBI/Uniprot data below describe general gene information for mCNTF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
22,587 Da
NCBI Official Full Name
ciliary neurotrophic factor
NCBI Official Synonym Full Names
ciliary neurotrophic factor
NCBI Official Symbol
NCBI Official Synonym Symbols
AI429687
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NCBI Protein Information
ciliary neurotrophic factor
UniProt Protein Name
Ciliary neurotrophic factor
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
CNTF_MOUSE
NCBI Summary for mCNTF
The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes, and it may be involved in reducing tissue destruction during inflammatory attacks. A read-through transcript variant composed of Zfp91 and Cntf sequences has been identified, but it is thought to be non-coding. Read-through transcription of Zfp91 and Cntf has been observed in both human and mouse. [provided by RefSeq, Aug 2008]
UniProt Comments for mCNTF
CNTF: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. Belongs to the CNTF family.

Cellular Component: extracellular space; cell soma; axon; cytoplasm; nucleus; cytosol

Molecular Function: interleukin-6 receptor binding; growth factor activity; cytokine activity; ciliary neurotrophic factor receptor binding

Biological Process: negative regulation of photoreceptor cell differentiation; nervous system development; positive regulation of axon regeneration; protein heterooligomerization; multicellular organismal development; membrane hyperpolarization; positive regulation of hormone secretion; positive regulation of JAK-STAT cascade; JAK-STAT cascade; positive regulation of cell growth; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; regulation of resting membrane potential; regulation of retinal cell programmed cell death; neuron development; negative regulation of neuron apoptosis; astrocyte activation; cell differentiation; muscle morphogenesis; growth
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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