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MCP-1 elisa kit :: Porcine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA Kit

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Catalog # MBS734478
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Monocyte chemotactic protein 1/monocyte chemotactic and activating factor (MCP-1), ELISA Kit

Popular Item
Full Product Name

Porcine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 607948
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Detection Range
1.0-25ng/mL
Sensitivity
0.1ng/mL
Intended Uses
This MCP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine MCP-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MCP-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for MCP-1purchase
MBS734478 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Monocyte chemotactic protein 1/monocyte chemotactic and activating factor (MCP-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MCP-1. The ELISA analytical biochemical technique of the MBS734478 kit is based on MCP-1 antibody-MCP-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MCP-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MCP-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MCP-1 elisa kit
Principle of the Assay: MCP-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MCP-1 antibody and an MCP-1-HRP conjugate. The assay sample and buffer are incubated together with MCP-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MCP-1 concentration since MCP-1 from samples and MCP-1-HRP conjugate compete for the anti-MCP-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MCP-1 from the sample, fewer sites are left to bind MCP-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MCP-1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for MCP-1 elisa kit

Typical Testing Data/Standard Curve (for reference only) of MCP-1 elisa kit
MCP-1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS734478. Click to request current manual
NCBI/Uniprot data below describe general gene information for MCP-1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
11,025 Da
NCBI Official Full Name
MCP-1
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 2
NCBI Official Symbol
NCBI Official Synonym Symbols
HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
  [Similar Products]
NCBI Protein Information
C-C motif chemokine 2; small-inducible cytokine A2; monocyte secretory protein JE; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)
UniProt Protein Name
C-C motif chemokine 2
UniProt Synonym Protein Names
HC11; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; MCP-1; Monocyte secretory protein JE; Small-inducible cytokine A2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
MCP1; SCYA2; MCAF; MCP-1  [Similar Products]
UniProt Entry Name
CCL2_HUMAN
NCBI Summary for MCP-1
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]
UniProt Comments for MCP-1
CCL2: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Chemokine

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space; rough endoplasmic reticulum; endocytic vesicle; perinuclear region of cytoplasm; dendrite; extracellular region; perikaryon; synapse; nerve terminal

Molecular Function: heparin binding; chemokine activity; CCR2 chemokine receptor binding; protein kinase activity; receptor binding

Biological Process: maternal process involved in pregnancy; protein amino acid phosphorylation; response to antibiotic; regulation of cell shape; monocyte chemotaxis; cell surface receptor linked signal transduction; transforming growth factor beta receptor signaling pathway; response to vitamin B3; negative regulation of neuron apoptosis; cellular homeostasis; cell adhesion; neutrophil chemotaxis; organ regeneration; positive regulation of tumor necrosis factor production; response to amino acid stimulus; JAK-STAT cascade; G-protein signaling, coupled to cyclic nucleotide second messenger; organ morphogenesis; response to ethanol; unfolded protein response, activation of signaling protein activity; cellular response to insulin stimulus; response to bacterium; response to heat; response to mechanical stimulus; positive regulation of endothelial cell proliferation; response to activity; response to progesterone stimulus; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of collagen biosynthetic process; chemotaxis; signal transduction; positive regulation of synaptic transmission; positive regulation of cellular extravasation; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; response to wounding; response to gamma radiation; angiogenesis; inflammatory response; lymphocyte chemotaxis; aging; unfolded protein response; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; cytoskeleton organization and biogenesis; viral genome replication; macrophage chemotaxis; humoral immune response; leukocyte migration during inflammatory response; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; positive regulation of leukocyte mediated cytotoxicity; cellular protein metabolic process; maternal process involved in parturition; response to hypoxia; positive regulation of T cell activation; vascular endothelial growth factor receptor signaling pathway; astrocyte cell migration

Disease: Neural Tube Defects; Mycobacterium Tuberculosis, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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