• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

mdeA recombinant protein :: L-Methionine gamma-Lyase Recombinant Protein

Scan QR to view Datasheet
Catalog # MBS844459
Unit / Price
  0.1 mg  /  $155 +1 FREE 8GB USB
  0.5 mg  /  $345 +1 FREE 8GB USB
Product Name

L-Methionine gamma-Lyase (mdeA), Recombinant Protein

Popular Item
Also Known As

L-Methionine gamma-Lyase, Pseudomonas putida recombinant

Product Synonym Names
L-Methionine-alpha-deamino-gamma-mercaptomethane-Lyase; Methioninase; METase; MGL; mdeA
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
398
3D Structure
ModBase 3D Structure for P13254
Host
E Coli
Purity/Purification
>=90%
Form/Format
Lyophilized from 10 mg/ml in 20 mM potassium phosphate, 150 mM NaCl, pH 8.3.
Appearance: Lyophilized
Solubility/Reconstitution Instructions
Briefly spin down and reconstitute in water or phosphate buffer.
Specific Activity
> or = 0.5 U/mg
Unit Definition
One unit is defined as the amount of enzyme required to convert 1.0 mumole of L-methionine to alpha-ketobutyrate, methanethiol and ammonia per minute in 20 mM potassium phosphate, pH 8.3, 150 mM NaCl at 37 degree C
Handling
Centrifuge the vial prior to opening.
Preparation and Storage
Store at –20 dergree C. Stable for at least 1 year as supplied. Avoid repeated freeze and thaw cycles.
Other Notes
Small volumes of mdeA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
mdeA recombinant protein
Background: Methionine gamma-lyase (EC 4.4.1.11) from Pseudomonas putida is a PLP-dependent enzyme which plays a central role in sulfur amino acid metabolism. METase catalyzes the alpha, gamma-elimination of methionine to alpha-ketobutyrate, methanethiol, and ammonia. METase also catalyzes the alpha, gamma-elimination of other sulfur containing amino acids, such as homocysteine, cysteine. Because of its ability to deplete methionine, METase has been considered as a viable component of cancer therapeutics against methionine-dependent tumor cells. METase has also been utilized to design drug targets for the infectious diseases caused by parasitic protozoa and anaerobic periodontal bacteria.
Applications Tested/Suitable for mdeA recombinant protein
SDS-PAGE; HPLC
Application Notes for mdeA recombinant protein
Recombinant bacterial Methionine Gamma-lyase can be used in inhibitor screening assays, activity studies, selectivity profiling, western blotting, ELISA, and numerous similar applications.

NCBI/Uniprot data below describe general gene information for mdeA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
UniProt Primary Accession #
Molecular Weight
47.1 kDa (1-398 aa, NT Poly-His Tag)
NCBI Official Full Name
Methionine gamma-lyase
UniProt Protein Name
Methionine gamma-lyase
UniProt Synonym Protein Names
L-methioninase
Protein Family
UniProt Gene Name
UniProt Entry Name
MEGL_PSEPU
UniProt Comments for mdeA
Catalyzes the alpha,gamma-elimination of L-methionine to produce methanethiol, 2-oxobutanoate and ammonia (PubMed:8586629, PubMed:6742420). Is involved in L-methionine catabolism (PubMed:9190812). In fact, shows a multicatalytic function since it also catalyzes gamma-replacement of L-methionine with thiol compounds, alpha,gamma-elimination and gamma-replacement reactions of L-homocysteine and its S-substituted derivatives, O-substituted-L-homoserines and DL-selenomethionine, and, to a lesser extent, alpha,beta-elimination and beta-replacement reactions of L-cysteine, S-methyl-L-cysteine, and O-acetyl-L-serine (PubMed:6742420, PubMed:22785484). Also catalyzes deamination and gamma-addition reactions of L-vinylglycine (PubMed:6742420). Thus, the enzyme is able to cleave C-S, C-Se, and C-O bonds of sulfur, selenium, and oxygen amino acids, respectively (PubMed:6742420, PubMed:22785484).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000