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MDH recombinant protein

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Catalog # MBS8249437
Unit / Price
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  0.01 mg  /  $255 +1 FREE 8GB USB
  0.05 mg  /  $635 +1 FREE 8GB USB
  0.5 mg  /  $2,370 +2 FREE 8GB USB
MDH recombinant protein
Product Name

MDH, Recombinant Protein

Full Product Name

Recombinant E Coli MDH Protein

Product Synonym Names
Malate dehydrogenase
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MGSSHHHHHH SSGLVPRGSH MKVAVLGAAG GIGQALALLL KTQLPSGSEL SLYDIAPVTP GVAVDLSHIP TAVKIKGFSG EDATPALEGA DVVLISAGVR RKPGMDRSDL FNVNAGIVKN LVQQVAKTCP KACIGIITNP VNTTVAIAAE VLKKAGVYDK NKLLGVTTLD IIRSNTFVAE LKGKQPGEVE VPVIGGHSGV TILPLLSQVP GVSFTEQEVA DLTKRIQNAG TEVVEAKAGG GSATLSMGQA AARFGLSLVR ALQGEQGVVE CAYVEGDGQY ARFFSQPLLL GKNGVEERKS IGTLSAFEQN ALEGMLDTLK KDIALGQEFV NK
3D Structure
ModBase 3D Structure for P61889
Host
E Coli
Species Reactivity
E Coli
Purity/Purification
Greater than 95% as determined by reducing SDS-PAGE.
Form/Format
Supplied as a 0.2 muM filtered solution of 50mM PB, 50% Glycerol, pH 7.5.
Endotoxin
Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
Directions for Use
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Preparation and Storage
Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.
Other Notes
Small volumes of MDH recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MDH recombinant protein
Recombinant E Coli MDH Protein is produced by our E Coli expression system and the target gene encoding Met1-Lys312 is expressed with a 6His tag at the N-terminus.
Applications Tested/Suitable for MDH recombinant protein
ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
NCBI/Uniprot data below describe general gene information for MDH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
Molecular Weight
32,337 Da
NCBI Official Full Name
malate dehydrogenase, NAD(P)-binding
NCBI Official Symbol
NCBI Official Synonym Symbols
ECK3225; JW3205
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NCBI Protein Information
malate dehydrogenase, NAD(P)-binding
UniProt Protein Name
Malate dehydrogenase
Protein Family
UniProt Gene Name
UniProt Entry Name
MDH_ECOLI
NCBI Summary for MDH
Mdh is elevated 4X during growth at 4C (enabled by Oleispira antarctica Cpn10/60 transgene). [More information is available at EcoGene: EG10576]. Malate dehydrogenase (Mdh) catalyzes the reversible oxidation of malate to generate oxaloacetate. [More information is available at EcoCyc: EG10576].
UniProt Comments for MDH
Catalyzes the reversible oxidation of malate to oxaloacetate.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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