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MDH1 recombinant protein :: Malate Dehydrogenase 1 Recombinant Protein

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Catalog # MBS142414
Unit / Price
  1000 IU  /  $140 +1 FREE 8GB USB
  5000 IU  /  $205 +1 FREE 8GB USB
  50000 IU  /  $1,020 +1 FREE 8GB USB
MDH1 recombinant protein
Product Name

Malate Dehydrogenase 1 (MDH1), Recombinant Protein

Popular Item
Full Product Name

Recombinant Malate Dehydrogenase 1

Product Synonym Names
MDH1 Chicken; Malate Dehydrogenase Chicken Recombinant; Malate dehydrogenase cytoplasmic; EC 1.1.1.37; Cytosolic malate dehydrogenase; MDHA; MOR2; MDH-s; MGC:1375; MDH1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q5ZME2
Host
E Coli
Purity/Purification
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Form/Format
Each mg of protein contains 0.59mg NaPO4.
Sterile lyophilized powder.
Unit Defenition
One unit is defined as 1 umol of NAD+ production per minute under the assay conditions (25 degree C, pH 7.5).
Specific Activity
Specific Activity Greater than 710U/mg protein.
Solubility
It is recommended to reconstitute the lyophilized Malate dehydrogenase in sterile 18M Omega -cm H2O.
Preparation and Storage
Lyophilized Malate dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution MDH1 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.
Other Notes
Small volumes of MDH1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MDH1 recombinant protein
Description: The DNA encoding Malate (Malic) Dehydrogenase is cloned from cDNA library of chicken heart.The MDH1 is purified by proprietary chromatographic techniques.

Introduction: Malate dehydrogenase (EC1.1.1.37) is an enzyme in the citric acid cycle that catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Malate dehydrogenase is not to be confused with malic enzyme, which catalyzes the conversion of pyruvate using NADPH.Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Pyruvate in the mitochondria is acted upon by pyruvate carboxylase to form oxaloacetate, a citric acid cycle intermediate. In order to get the oxaloacetate out of the mitochondria, malate dehydrogenase reduces it to malate, and it then traverses the inner mitochondrial membrane. Once in the cytosol, the malate is oxidized back to oxaloacetate by cytosolic malate dehydrogenase. Finally, phosphoenol-pyruvate carboxy kinase (PEPCK) converts oxaloacetate to phosphoenol pyruvate.
NCBI/Uniprot data below describe general gene information for MDH1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
36,543 Da
NCBI Official Full Name
malate dehydrogenase, cytoplasmic
NCBI Official Synonym Full Names
malate dehydrogenase 1, NAD (soluble)
NCBI Official Symbol
NCBI Protein Information
malate dehydrogenase, cytoplasmic; cytosolic malate dehydrogenase
UniProt Protein Name
Malate dehydrogenase, cytoplasmic
UniProt Synonym Protein Names
Cytosolic malate dehydrogenase
Protein Family
UniProt Gene Name
UniProt Entry Name
MDHC_CHICK
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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