NP_001275515.1
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NCBI GenBank Nucleotide #
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UniProt Primary Accession #
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UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
56.6 kDa
NCBI Official Full Name
E3 ubiquitin-protein ligase Mdm2
NCBI Official Synonym Full Names
transformed mouse 3T3 cell double minute 2
NCBI Protein Information
E3 ubiquitin-protein ligase Mdm2
UniProt Protein Name
E3 ubiquitin-protein ligase Mdm2
UniProt Synonym Protein Names
Double minute 2 protein; Oncoprotein Mdm2; p53-binding protein Mdm2
UniProt Entry Name
MDM2_MOUSE
UniProt Comments for Mdm2
MDM2: a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 via Akt-mediated MDM2 phosphorylation. Phosphorylation of MDM2 increases its interaction with p300, providing a platform to allow the assembly of the protein complex necessary for MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. Facilitates the nuclear export of p53 and targets it for proteasome-mediated proteolysis. Eight alternatively spliced isoforms have been reported.
Protein type: Oncoprotein; Nucleolus; Inhibitor; EC 6.3.2.19; Ligase; Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system
Cellular Component: cytoplasm; cytosol; nuclear body; nucleolus; nucleoplasm; nucleus; plasma membrane; protein complex; synapse
Molecular Function: coenzyme F420-0 gamma-glutamyl ligase activity; coenzyme F420-2 alpha-glutamyl ligase activity; enzyme binding; identical protein binding; ligase activity; metal ion binding; p53 binding; peroxisome proliferator activated receptor binding; protein binding; ribosomal S6-glutamic acid ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; zinc ion binding
Biological Process: blood vessel development; blood vessel remodeling; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; establishment of protein localization; heart development; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; peptidyl-lysine modification; positive regulation of cell cycle; positive regulation of mitotic cell cycle; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein export from nucleus; protein complex assembly; protein destabilization; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of gene expression; regulation of heart rate; regulation of protein catabolic process; traversing start control point of mitotic cell cycle
Product References and Citations for Mdm2 recombinant protein
Tumorigenic potential associated with enhanced expression of a gene that is amplified in a mouse tumor cell line.Fakharzadeh S.S., Trusko S.P., George D.L.EMBO J. 10:1565-1569(1991)
Genomic organization of the mouse double minute 2 gene.Jones S.N., Ansari-Lari M.A., Hancock A.R., Jones W.J., Gibbs R.A., Donehower L.A., Bradley A.Gene 175:209-213(1996)
The organization and expression of the mdm2 gene.de Oca Luna R.M., Tabor A.D., Eberspaecher H., Hulboy D.L., Worth L.L., Colman M.S., Finlay C.A., Lozano G.Genomics 33:352-357(1996)
Multiple murine double minute gene 2 (MDM2)
proteins are induced by ultraviolet light.Saucedo L.J., Myers C.D., Perry M.E.J. Biol. Chem. 274:8161-8168(1999)
Cooperative signals governing ARF-mdm2 interaction and nucleolar localization of the complex.Weber J.D., Kuo M.-L., Bothner B., DiGiammarino E.L., Kriwacki R.W., Roussel M.F., Sherr C.J.Mol. Cell. Biol. 20:2517-2528(2000)
Rapid ATM-dependent phosphorylation of MDM2 precedes p53 accumulation in response to DNA damage.Khosravi R., Maya R., Gottlieb T., Oren M., Shiloh Y., Shkedy D.Proc. Natl. Acad. Sci. U.S.A. 96:14973-14977(1999)
A novel cellular protein (MTBP)
binds to MDM2 and induces a G1 arrest that is suppressed by MDM2.Boyd M.T., Vlatkovic N., Haines D.S.J. Biol. Chem. 275:31883-31890(2000)
Regulation of receptor fate by ubiquitination of activated beta 2-adrenergic receptor and beta-arrestin.Shenoy S.K., McDonald P.H., Kohout T.A., Lefkowitz R.J.Science 294:1307-1313(2001)
PML regulates p53 stability by sequestering Mdm2 to the nucleolus.Bernardi R., Scaglioni P.P., Bergmann S., Horn H.F., Vousden K.H., Pandolfi P.P.Nat. Cell Biol. 6:665-672(2004)
A novel nuclear interactor of ARF and MDM2 (NIAM)
that maintains chromosomal stability.Tompkins V.S., Hagen J., Frazier A.A., Lushnikova T., Fitzgerald M.P., di Tommaso A.D., Ladeveze V., Domann F.E., Eischen C.M., Quelle D.E.J. Biol. Chem. 282:1322-1333(2007)
Research Articles on Mdm2
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Products associated with Mdm2 recombinant protein
Pathways associated with Mdm2 recombinant protein
Diseases associated with Mdm2 recombinant protein
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