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MFAP5 elisa kit :: Mouse microfibrillar associated protein 5 ELISA Kit

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Catalog # MBS910817
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Typical Testing Data/Standard Curve (for reference only)
Product Name

microfibrillar associated protein 5 (MFAP5), ELISA Kit

Popular Item
Also Known As

Mouse Microfibrillar-associated protein 5, MFAP5 ELISA Kit

Product Synonym Names
Mouse Microfibrillar-associated protein 5 (MFAP5) ELISA kit; MAGP2; MP25; microfibril-associated glycoprotein 2; microfibril-associated glycoprotein-2; microfibrillar associated protein 5
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS910817 Testing Data
Sequence Length
164
3D Structure
ModBase 3D Structure for Q9QZJ6
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse MFAP5. No significant cross-reactivity or interference between mouse MFAP5 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
0.156 ng/ml -10 ng/ml.
Sensitivity
The minimum detectable dose of mouse MFAP5 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MFAP5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forMFAP5purchase
MBS910817 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the microfibrillar associated protein 5 (MFAP5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MFAP5. The ELISA analytical biochemical technique of the MBS910817 kit is based on MFAP5 antibody-MFAP5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MFAP5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MFAP5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Research into extracellular matrix glycoproteins, such as microfibrillar associated protein 5 - 5 MFAP5 - have revealed novel roles in human disease. Mouse models require quantitative abilities for microfibrillar protein (MFAP), including MFAP5 ELISA for these glycoprotein studies. Manufactured by Promega or Roche Applied Science and distributed by MyBiosource, this MFAP5 ELISA kit product is ideal for cell samples detection of protein 5 MFAP5 for comparative cell proliferation assay. See assay system Promega for luciferase assay. Microfibrillar protein 5 - 5 MFAP5 ELISA is highly specific for microfibrillar protein 5 and will not cross react with MAGP 2, microfibrillar protein 4 - protein 4 MFAP4 - human microfibrillar protein or other microfibril glycoprotein products. Besides this detection kit, we offer sheep, rat, pig or monkey ELISA kits with the same high quality primary antibody and secondary antibodies. View kit microfibrillar protein to order for non diagnostic use only.
Related Product Information for
MFAP5 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MFAP5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MFAP5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MFAP5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MFAP5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of MFAP5 elisa kit
MFAP5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS910817. Click to request current manual
NCBI/Uniprot data below describe general gene information for MFAP5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
18,538 Da
NCBI Official Full Name
microfibrillar-associated protein 5
NCBI Official Synonym Full Names
microfibrillar associated protein 5
NCBI Official Symbol
Mfap5  [Similar Products]
NCBI Official Synonym Symbols
MAGP-2
  [Similar Products]
NCBI Protein Information
microfibrillar-associated protein 5; MFAP-5; microfibril-associated glycoprotein 2
UniProt Protein Name
Microfibrillar-associated protein 5
UniProt Synonym Protein Names
Microfibril-associated glycoprotein 2; MAGP-2
UniProt Gene Name
Mfap5  [Similar Products]
UniProt Synonym Gene Names
Magp2; MFAP-5; MAGP-2  [Similar Products]
UniProt Entry Name
MFAP5_MOUSE
UniProt Comments for MFAP5
MFAP5: Component of the elastin-associated microfibrils. Belongs to the MFAP family.

Protein type: Secreted, signal peptide; Extracellular matrix; Secreted

Cellular Component: proteinaceous extracellular matrix; extracellular region; microfibril

Molecular Function: extracellular matrix constituent conferring elasticity

Biological Process: fibril organization and biogenesis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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