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MFGE8 elisa kit :: Bovine Lactadherin (MFGE8) ELISA Kit

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Catalog # MBS756386
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Lactadherin (MFGE8), ELISA Kit

Popular Item
Full Product Name

Bovine Lactadherin (MFGE8) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
602281
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of MFGE8. No significant cross-reactivity or interference between MFGE8 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MFGE8 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
100-2500pg/ml
Sensitivity
1.0pg/ml
Intended Uses
This MFGE8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine MFGE8. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MFGE8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for MFGE8purchase
MBS756386 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lactadherin (MFGE8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MFGE8. The ELISA analytical biochemical technique of the MBS756386 kit is based on MFGE8 antibody-MFGE8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MFGE8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MFGE8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MFGE8 elisa kit
Principle of the assay: MFGE8 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MFGE8. Standards or samples are then added to the microtiter plate wells and MFGE8 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MFGE8 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MFGE8 are added to each well to “sandwich” the MFGE8 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MFGE8 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MFGE8 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for MFGE8 elisa kit

Typical Testing Data/Standard Curve (for reference only) of MFGE8 elisa kit
MFGE8 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS756386. Click to request current manual
NCBI/Uniprot data below describe general gene information for MFGE8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
37,523 Da
NCBI Official Full Name
lactadherin isoform a preproprotein
NCBI Official Synonym Full Names
milk fat globule-EGF factor 8 protein
NCBI Official Symbol
MFGE8  [Similar Products]
NCBI Official Synonym Symbols
BA46; HMFG; MFGM; SED1; hP47; EDIL1; MFG-E8; SPAG10; OAcGD3S; HsT19888
  [Similar Products]
NCBI Protein Information
lactadherin; medin; sperm surface protein hP47; sperm associated antigen 10; breast epithelial antigen BA46; O-acetyl disialoganglioside synthase
UniProt Protein Name
Lactadherin
UniProt Synonym Protein Names
Breast epithelial antigen BA46; HMFG; MFGM; Milk fat globule-EGF factor 8; MFG-E8; SED1Cleaved into the following 2 chains:Lactadherin short form; Medin
Protein Family
UniProt Gene Name
MFGE8  [Similar Products]
UniProt Synonym Gene Names
MFG-E8  [Similar Products]
UniProt Entry Name
MFGM_HUMAN
NCBI Summary for MFGE8
This gene encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid (AMA). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
UniProt Comments for MFGE8
MFGE8: Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization. Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 15q25

Cellular Component: extrinsic to plasma membrane; extracellular matrix; extracellular space; membrane; extracellular region; vesicle; external side of plasma membrane

Molecular Function: integrin binding; phosphatidylethanolamine binding; phosphatidylserine binding

Biological Process: viral reproduction; single fertilization; phagocytosis, recognition; angiogenesis; cell adhesion; phagocytosis, engulfment
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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