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MIA recombinant protein :: Melanoma Inhibitory Activity Recombinant Protein

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Catalog # MBS142187
Unit / Price
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  0.005 mg  /  $140 +1 FREE 8GB USB
  0.02 mg  /  $205 +1 FREE 8GB USB
  1 mg  /  $3,435 +4 FREE 8GB USB
MIA recombinant protein
Product Name

Melanoma Inhibitory Activity (MIA), Recombinant Protein

Full Product Name

Recombinant Human Melanoma Inhibitory Activity Protein

Product Synonym Names
MIA Human; Melanoma Inhibitory Activity Human Recombinant; Melanoma-derived growth regulatory protein precursor; Cartilage-derived retinoic acid-sensitive protein; CD-RAP; MIA
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
Agrees with the sequence of native MIA human with an addition N-terminal Methionine residue.MG PMPKLADRKL CADQECSSHP ISMAVALQDY MAPDCRFLTI HRGQVVYVFS LKGRGRFLWG GSVQGDYYGD LAARLGYFPS SIVREDQTLK VDVKTDKWDF YCQ
OMIM
601340
3D Structure
ModBase 3D Structure for Q16674
Host
E Coli
Purity/Purification
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Form/Format
The protein was lyophilized from a concentrated (1mg/ml) solution containing 20mM Potassium-phosphate pH=7 and 150mM potassium chloride.
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the lyophilized Melanoma Inhibitory Activity in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
UV spectroscopy at 280 nm using the absorption coefficient of 19300 M-1cm-1.
Biological Activity
The biological activity is calculated by the inhibiting effect on the invasion of Mel In Tumor cells and found active in Mel In assay.
Preparation and Storage
Lyophilized MIA although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution MIA should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.
Other Notes
Small volumes of MIA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MIA recombinant protein
Description: Melanoma Inhibitory Activity Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain consisting of 108 amino having a total molecular mass of 12237 Dalton.The MIA is purified by proprietary chromatographic techniques.

Introduction: The Melanoma Inhibitory protein (MIA) was identified as an inhibitor of in vitro growth of malignant melanoma cells. The protein contains a SH3 domain. MIA acts as a potent tumor cell growth inhibitor for malignant melanoma cells and some other neuroectodermal tumors, including gliomas, in an autocrine fashion. In a study of human melanoma cell lines with different metastatic capacity MIA mRNA expression appeared to be inversely correlated with pigmentation. MIA has been shown to represent a very sensitive and specific serum marker for systemic malignant melanoma that might be useful for staging of primary melanomas, detection of progression from localized to metastatic disease during follow-up, and monitoring therapy of advanced melanomas.
NCBI/Uniprot data below describe general gene information for MIA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
6,361 Da
NCBI Official Full Name
melanoma-derived growth regulatory protein
NCBI Official Synonym Full Names
melanoma inhibitory activity
NCBI Official Symbol
NCBI Official Synonym Symbols
CD-RAP
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NCBI Protein Information
melanoma-derived growth regulatory protein
UniProt Protein Name
Melanoma-derived growth regulatory protein
UniProt Synonym Protein Names
Melanoma inhibitory activity protein
UniProt Gene Name
UniProt Entry Name
MIA_HUMAN
UniProt Comments for MIA
MIA: Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas. Belongs to the MIA/OTOR family.

Protein type: Cell adhesion; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space

Molecular Function: growth factor activity

Biological Process: cell proliferation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with MIA recombinant protein
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