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MIP-3beta/ELC/CCL19 elisa kit :: Mouse MIP-3beta ELISA Kit

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Catalog # MBS2501961
Unit / Price
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  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

MIP-3beta (MIP-3beta/ELC/CCL19), ELISA Kit

Popular Item
Full Product Name

Mouse MIP-3beta(Macrophage Inflammatory Protein 3beta) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
602227
3D Structure
ModBase 3D Structure for Q99731
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse MIP-3beta/ELC/CCL19. No significant cross-reactivity or interference between Mouse MIP-3beta/ELC/CCL19 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse MIP-3beta/ELC/CCL19 and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
15.625-1000pg/mL
Sensitivity
Min: 9.375pg/mL; Max: 1000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MIP-3beta/ELC/CCL19 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forMIP-3beta/ELC/CCL19purchase
MBS2501961 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MIP-3beta (MIP-3beta/ELC/CCL19) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MIP-3beta/ELC/CCL19. The ELISA analytical biochemical technique of the MBS2501961 kit is based on MIP-3beta/ELC/CCL19 antibody-MIP-3beta/ELC/CCL19 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MIP-3beta/ELC/CCL19 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MIP-3beta/ELC/CCL19. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MIP-3beta/ELC/CCL19 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MIP-3beta/ELC/CCL19 in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MIP-3beta/ELC/CCL19. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse MIP-3beta/ELC/CCL19 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MIP-3beta/ELC/CCL19, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse MIP-3beta/ELC/CCL19. You can calculate the concentration of Mouse MIP-3beta/ELC/CCL19 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of MIP-3beta/ELC/CCL19 elisa kit
MIP-3beta/ELC/CCL19 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2501961. Click to request current manual
NCBI/Uniprot data below describe general gene information for MIP-3beta/ELC/CCL19. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
10,993 Da
NCBI Official Full Name
macrophage inflammatory protein 3 beta
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 19
NCBI Official Symbol
CCL19  [Similar Products]
NCBI Official Synonym Symbols
ELC; CKb11; MIP3B; MIP-3b; SCYA19
  [Similar Products]
NCBI Protein Information
C-C motif chemokine 19; CC chemokine ligand 19; CK beta-11; EBI1 ligand chemokine; EBI1-ligand chemokine; MIP-3-beta; beta chemokine exodus-3; beta-chemokine exodus-3; epstein-Barr virus-induced molecule 1 ligand chemokine; macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; small inducible cytokine subfamily A (Cys-Cys), member 19; small-inducible cytokine A19
UniProt Protein Name
C-C motif chemokine 19
UniProt Synonym Protein Names
Beta-chemokine exodus-3; CK beta-11; Epstein-Barr virus-induced molecule 1 ligand chemokine; EBI1 ligand chemokine; ELC; Macrophage inflammatory protein 3 beta; MIP-3-beta; Small-inducible cytokine A19
UniProt Gene Name
CCL19  [Similar Products]
UniProt Synonym Gene Names
ELC; MIP3B; SCYA19; EBI1 ligand chemokine; ELC; MIP-3-beta  [Similar Products]
UniProt Entry Name
CCL19_HUMAN
NCBI Summary for MIP-3beta/ELC/CCL19
This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7. [provided by RefSeq, Sep 2014]
UniProt Comments for MIP-3beta/ELC/CCL19
CCL19: May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: extracellular space; extracellular region

Molecular Function: CCR chemokine receptor binding; chemokine activity; chemokine receptor binding; CCR10 chemokine receptor binding; CCR7 chemokine receptor binding

Biological Process: formation of immunological synapse; positive regulation of interleukin-12 production; positive regulation of dendritic cell antigen processing and presentation; cell maturation; positive regulation of JNK cascade; positive regulation of endocytosis; positive regulation of NF-kappaB import into nucleus; positive regulation of interleukin-1 beta secretion; activation of JNK activity; positive regulation of receptor-mediated endocytosis; dendritic cell chemotaxis; cell communication; positive regulation of T cell proliferation; establishment of T cell polarity; inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of chemotaxis; response to virus; positive regulation of phosphoinositide 3-kinase activity; positive regulation of tumor necrosis factor production; cellular calcium ion homeostasis; positive regulation of protein kinase B signaling cascade; myeloid dendritic cell chemotaxis; positive regulation of protein kinase activity; release of sequestered calcium ion into cytosol; T cell costimulation; immune response; positive regulation of T-helper 1 cell differentiation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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