• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

MIP3beta/ELC/CCL19 elisa kit :: Rat macrophage inflammatory protein 3beta, MIP-3beta ELISA Kit

Scan QR to view Datasheet
Catalog # MBS701119
Unit / Price
Scan QR to view Datasheet
DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Typical Testing Data/Standard Curve (for reference only)
Product Name

macrophage inflammatory protein 3beta, MIP-3beta (MIP3beta/ELC/CCL19), ELISA Kit

Full Product Name

Rat macrophage inflammatory protein 3beta, MIP-3beta ELISA Kit

Product Synonym Names
Rat macrophage inflammatory protein 3beta; MIP-3beta ELISA kit; macrophage inflammatory protein 3beta; MIP-3beta
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
602227
3D Structure
ModBase 3D Structure for Q99731
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of rat MIP-3beta. No significant cross-reactivity or interference between rat MIP-3beta and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
12.5 pg/ml -800 pg/ml.
Sensitivity
The minimum detectable dose of rat MIP-3beta is typically less than 3.12 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MIP3beta/ELC/CCL19 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for MIP3beta/ELC/CCL19purchase
MBS701119 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the macrophage inflammatory protein 3beta, MIP-3beta (MIP3beta/ELC/CCL19) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MIP3beta/ELC/CCL19. The ELISA analytical biochemical technique of the MBS701119 kit is based on MIP3beta/ELC/CCL19 antibody-MIP3beta/ELC/CCL19 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MIP3beta/ELC/CCL19 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MIP3beta/ELC/CCL19. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MIP3beta/ELC/CCL19 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MIP-3beta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIP-3beta present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIP-3beta is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIP-3beta bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of MIP3beta/ELC/CCL19 elisa kit
MIP3beta/ELC/CCL19 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS701119. Click to request current manual
NCBI/Uniprot data below describe general gene information for MIP3beta/ELC/CCL19. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
10,993 Da
NCBI Official Full Name
macrophage inflammatory protein 3 beta
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 19
NCBI Official Symbol
CCL19  [Similar Products]
NCBI Official Synonym Symbols
ELC; CKb11; MIP3B; MIP-3b; SCYA19
  [Similar Products]
NCBI Protein Information
C-C motif chemokine 19; exodus-3; CK beta-11; MIP-3-beta; EBI1 ligand chemokine; EBI1-ligand chemokine; CC chemokine ligand 19; beta chemokine exodus-3; beta-chemokine exodus-3; small-inducible cytokine A19; macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; epstein-Barr virus-induced molecule 1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19
UniProt Protein Name
C-C motif chemokine 19
UniProt Synonym Protein Names
Beta-chemokine exodus-3; CK beta-11; Epstein-Barr virus-induced molecule 1 ligand chemokine; EBI1 ligand chemokine; ELC; Macrophage inflammatory protein 3 beta; MIP-3-beta; Small-inducible cytokine A19
UniProt Gene Name
CCL19  [Similar Products]
UniProt Synonym Gene Names
ELC; MIP3B; SCYA19; EBI1 ligand chemokine; ELC; MIP-3-beta  [Similar Products]
UniProt Entry Name
CCL19_HUMAN
NCBI Summary for MIP3beta/ELC/CCL19
This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7. [provided by RefSeq, Jul 2008]
UniProt Comments for MIP3beta/ELC/CCL19
Function: May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.1 PublicationManual assertion based on experiment in:Ref.6
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000