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MMP-2 elisa kit :: Human MMP-2 ELISA Kit

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Catalog # MBS355315
Unit / Price
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  96 Tests  /  $465 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

MMP-2, ELISA Kit

Popular Item
Full Product Name

MMP-2 Human ELISA Kit

Product Synonym Names
Human MMP-2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 13; NC_018902.1 (5952853..5963249). Location: chromosome: 13
Species Reactivity
Assay Type
Sandwich
Detection Range
156 pg/ml-10,000 pg/ml
Sensitivity
< 10pg/ml
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MMP-2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for MMP-2purchase
MBS355315 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MMP-2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MMP-2. The ELISA analytical biochemical technique of the MBS355315 kit is based on MMP-2 antibody-MMP-2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP-2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP-2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MMP-2 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MMP-2 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MMP-2 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MMP-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MMP-2 can be calculated.

Background: Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72-kD. It is a metalloproteinase that specifically cleaves type IV collagen, the major structural component of basement membranes. MMP-2 is a likely effector of endometrial menstrual breakdown. It plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Kenny et al. (2008) found that expression of MMP2 was induced upon attachment of ovarian cancer (OvCa) cells to mesothelium.
Applications Tested/Suitable for MMP-2 elisa kit
Quantitative sELISA (EIA)
Application Notes for MMP-2 elisa kit
For quantitative detection of MMP-2 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.

Typical Testing Data/Standard Curve (for reference only) of MMP-2 elisa kit
MMP-2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS355315. Click to request current manual
NCBI/Uniprot data below describe general gene information for MMP-2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
matrix metalloproteinase-2
NCBI Official Symbol
mmp-2  [Similar Products]
NCBI Official Synonym Symbols
fgmmp-2
  [Similar Products]
NCBI Protein Information
matrix metalloproteinase-2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with MMP-2 elisa kit
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