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MMP-8 elisa kit :: Porcine Metalloproteinase 8 (MMP-8) ELISA Kit

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Catalog # MBS267821
Unit / Price
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  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $475 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Metalloproteinase 8 (MMP-8), ELISA Kit

Popular Item
Full Product Name

Porcine Metalloproteinase 8 (MMP-8) ELISA kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
602262
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant MMP-8 concentration
Assay Type
Sandwich
Detection Range
5000 pg/ml-78 pg/ml
Sensitivity
20 pg/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MMP-8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for MMP-8purchase
MBS267821 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Metalloproteinase 8 (MMP-8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MMP-8. The ELISA analytical biochemical technique of the MBS267821 kit is based on MMP-8 antibody-MMP-8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP-8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP-8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MMP-8 elisa kit
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine MMP-8 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of MMP-8 elisa kit
MMP-8 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS267821. Click to request current manual
NCBI/Uniprot data below describe general gene information for MMP-8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
52,515 Da
NCBI Official Full Name
metalloproteinase
NCBI Official Synonym Full Names
matrix metallopeptidase 16 (membrane-inserted)
NCBI Official Symbol
MMP16  [Similar Products]
NCBI Official Synonym Symbols
MMP-X2; C8orf57; MT-MMP2; MT-MMP3; MT3-MMP
  [Similar Products]
NCBI Protein Information
matrix metalloproteinase-16; MMP-16; MT3MMP; MTMMP3; MT-MMP 3; Putative transmembrane protein C8orf57; membrane-type matrix metalloproteinase 3; membrane-type-3 matrix metalloproteinase
UniProt Protein Name
Matrix metalloproteinase-16
UniProt Synonym Protein Names
MMP-X2; Membrane-type matrix metalloproteinase 3; MT-MMP 3; MTMMP3; Membrane-type-3 matrix metalloproteinase; MT3-MMP; MT3MMP
UniProt Gene Name
MMP16  [Similar Products]
UniProt Synonym Gene Names
MMPX2; MMP-16; MT-MMP 3; MTMMP3; MT3-MMP; MT3MMP  [Similar Products]
UniProt Entry Name
MMP16_HUMAN
NCBI Summary for MMP-8
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The encoded protein activates MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16. [provided by RefSeq, Oct 2010]
UniProt Comments for MMP-8
MMP16: Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells. Belongs to the peptidase M10A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.-; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q21.3

Cellular Component: proteinaceous extracellular matrix; cell surface; Golgi lumen; integral to plasma membrane; plasma membrane

Molecular Function: zinc ion binding; metalloendopeptidase activity; enzyme activator activity; calcium ion binding

Biological Process: positive regulation of catalytic activity; extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; embryonic cranial skeleton morphogenesis; proteolysis; endochondral ossification
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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