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MMP14 elisa kit :: Human MMP14 ELISA Kit

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Catalog # MBS178524
Unit / Price
  96-Strip-Wells  /  $425 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

MMP14, ELISA Kit

Popular Item
Also Known As

Human MMP-14 PicoKine ELISA Kit

Product Synonym Names
MMP 14; MMP14; MMP-14; MMP X1; MMPX1; MMP-X1; MT MMP 1; MT-MMP 1; MT1 MMP; MT1-MMP; MT1MMP; MTMMP 1; MTMMP1; P50281; Matrix metalloproteinase-14; matrix metallopeptidase 14
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
582
OMIM
277950
3D Structure
ModBase 3D Structure for P50281
Species Reactivity
Specificity
Natural and recombinant Human MMP-14
Assay Type
Quantitative Sandwich
Detection Range
156pg/ml-10000pg/ml
Sensitivity
<15pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MMP14 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forMMP14purchase
MBS178524 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MMP14, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MMP14. The ELISA analytical biochemical technique of the MBS178524 kit is based on MMP14 antibody-MMP14 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP14 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP14. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MMP14 elisa kit
Principle of the Assay: Human MMP-14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A specific for MMP-14 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: A21-S538) and test samples are added to the wells, a biotinylated detection specific for MMP-14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MMP-14 amount of sample captured in plate.

Background: Matrix metalloproteinase-14 is an enzyme that in humans is encoded by the MMP14 gene. It is mapped to chromosome 14q11-q12. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive pro-proteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted.

Typical Testing Data/Standard Curve (for reference only) of MMP14 elisa kit
MMP14 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS178524. Click to request current manual
NCBI/Uniprot data below describe general gene information for MMP14. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
65,894 Da
NCBI Official Full Name
matrix metalloproteinase-14 preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 14
NCBI Official Symbol
MMP14  [Similar Products]
NCBI Official Synonym Symbols
MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1
  [Similar Products]
NCBI Protein Information
matrix metalloproteinase-14
UniProt Protein Name
Matrix metalloproteinase-14
UniProt Synonym Protein Names
MMP-X1; Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; MT1-MMP; MT1MMP
Protein Family
UniProt Gene Name
MMP14  [Similar Products]
UniProt Synonym Gene Names
MMP-14; MT-MMP 1; MTMMP1; MT1-MMP; MT1MMP  [Similar Products]
UniProt Entry Name
MMP14_HUMAN
NCBI Summary for MMP14
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008]
UniProt Comments for MMP14
Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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