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MMP8 elisa kit :: Human MMP8 ELISA Kit

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Catalog # MBS824833
Unit / Price
  96 Tests  /  $470 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

MMP8, ELISA Kit

Popular Item
Full Product Name

Human MMP8 ELISA Kit

Product Synonym Names
CLG1; Neutrophil collagenase; Matrix metalloproteinase-8; MMP-8; PMNL collagenase; PMNL-CL
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
120355
3D Structure
ModBase 3D Structure for P22894
Species Reactivity
Specificity
The Human MMP8 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human MMP8 proteins within the range of 156 pg/ml - 10000 pg/ml.
Samples
Cell Culture Supernatants, Serum, Plasma
Sensitivity
The minimum detectable dose of Human MMP8 is typically less than 10 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MMP8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forMMP8purchase
MBS824833 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MMP8, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MMP8. The ELISA analytical biochemical technique of the MBS824833 kit is based on MMP8 antibody-MMP8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MMP8 elisa kit
Principle of the assay: The Bioscience Human MMP8 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP8 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human MMP8 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Matrix metalloproteinase 8 (MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase (HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation.
Applications Tested/Suitable for MMP8 elisa kit
Sandwich ELISA (SE)

Typical Testing Data/Standard Curve (for reference only) of MMP8 elisa kit
MMP8 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS824833. Click to request current manual
NCBI/Uniprot data below describe general gene information for MMP8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
53,412 Da
NCBI Official Full Name
neutrophil collagenase preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 8 (neutrophil collagenase)
NCBI Official Symbol
NCBI Official Synonym Symbols
HNC; CLG1; MMP-8; PMNL-CL
  [Similar Products]
NCBI Protein Information
neutrophil collagenase; PMNL collagenase; matrix metalloproteinase-8; matrix metalloproteinase 8 (neutrophil collagenase)
UniProt Protein Name
Neutrophil collagenase
UniProt Synonym Protein Names
Matrix metalloproteinase-8; MMP-8; PMNL collagenase; PMNL-CL
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CLG1; MMP-8; PMNL-CL  [Similar Products]
UniProt Entry Name
MMP8_HUMAN
NCBI Summary for MMP8
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]
UniProt Comments for MMP8
MMP8: Can degrade fibrillar type I, II, and III collagens. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.34; Protease; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: zinc ion binding; serine-type endopeptidase activity; metalloendopeptidase activity; calcium ion binding

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; proteolysis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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