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MMP8 elisa kit :: Mouse matrix metallopeptidase 8 (neutrophil collagenase) ELISA Kit

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Catalog # MBS703729
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $450 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,615 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,940 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

matrix metallopeptidase 8 (neutrophil collagenase) (MMP8), ELISA Kit

Popular Item
Also Known As

Mouse matrix metalloproteinase 8/Neutrophil collagenase, MMP-8 ELISA Kit

Product Synonym Names
Mouse matrix metalloproteinase 8/Neutrophil collagenase; MMP-8 ELISA kit; CLG1; HNC; PMNL-CL; PMNL collagenase; matrix metalloproteinase 8; matrix metalloproteinase 8 (neutrophil collagenase) ; matrix metallopeptidase 8 (neutrophil collagenase)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS703729 Testing Data
Sequence Length
465
3D Structure
ModBase 3D Structure for O70138
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse MMP-8. No significant cross-reactivity or interference between mouse MMP-8 and analogues was observed.
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Assay Type
Sandwich
Detection Range
15.6 pg/ml -1000 pg/ml.
Sensitivity
The minimum detectable dose of mouse MMP-8 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MMP8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forMMP8purchase
MBS703729 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the matrix metallopeptidase 8 (neutrophil collagenase) (MMP8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MMP8. The ELISA analytical biochemical technique of the MBS703729 kit is based on MMP8 antibody-MMP8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Mouse matrix metalloproteinase 8, also termed matrix metallopeptidase 8 MMP 8, is unusual in the family of collagenase proteins because it is sequestered in an active form and secreted by neutrophils into serum or plasma. Quantitative detection of mouse free serum plasma or cell culture supernatant neutrophil collagenase MMP8 is possible down to 3.9 pg ml with a sandwich format ELISA kit. The assay system Promega devised utilizes a pre bound polyclonal antibody, biotinylated secondary antibodies and flexible detection products for HRP, either colorimetric or Alexa 174 fluor. Recombinant matrix metalloproteinase 8 protein is included in the ELISA kits for standard curve on each plate, increasing assay precision for MMP8 neutrophil collagenase. Mouse assay kit is specific and does not cross react with MMP8 human or rat. Available in 48 tests or 96 tests formats to order for research purposes such as cell proliferation responses to metallopeptidase 8 neutrophil concentration.
Related Product Information for
MMP8 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of MMP8 elisa kit
MMP8 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS703729. Click to request current manual
NCBI/Uniprot data below describe general gene information for MMP8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
53,126 Da
NCBI Official Full Name
neutrophil collagenase
NCBI Official Synonym Full Names
matrix metallopeptidase 8
NCBI Official Symbol
NCBI Official Synonym Symbols
BB138268
  [Similar Products]
NCBI Protein Information
neutrophil collagenase; MMP-8; collagenase 2; collagenase-2; matrix metalloproteinase 8; matrix metalloproteinase-8
UniProt Protein Name
Neutrophil collagenase
UniProt Synonym Protein Names
Collagenase 2; Matrix metalloproteinase-8; MMP-8
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
MMP-8  [Similar Products]
UniProt Entry Name
MMP8_MOUSE
UniProt Comments for MMP8
Function: Can degrade fibrillar type I, II, and III collagens. May play a role in the degradation of collagen fibers during uterine involution.

Catalytic activity: Cleavage of interstitial collagens in the triple helical domain. Unlike EC 3.4.24.7, this enzyme cleaves type III collagen more slowly than type I.

Cofactor: Binds 3 calcium ions per subunit

Enzyme regulation: Cannot be activated without removal of the activation peptide. Activated by matrilysin.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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