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MMP8 recombinant protein :: Matrix Metalloproteinase 8 Recombinant Protein

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Catalog # MBS2031518 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $145 +1 FREE 8GB USB
  0.05 mg  /  $275 +1 FREE 8GB USB
  0.1 mg  /  $430 +1 FREE 8GB USB
  0.2 mg  /  $525 +1 FREE 8GB USB
  0.5 mg  /  $1,015 +1 FREE 8GB USB
  1 mg  /  $1,515 +1 FREE 8GB USB
SDS-Page
Product Name

Matrix Metalloproteinase 8 (MMP8), Recombinant Protein

Popular Item
Also Known As

Recombinant Matrix Metalloproteinase 8 (MMP8)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS2031518 Technical Datasheet
OMIM
120355
Host
E.Coli
Purity/Purification
> 90%
Concentration
Original Concentration: 200 Ug/mL (lot specific)
Organism Species
Rattus norvegicus (Rat)
Source
Prokaryotic expression
Residues
Leu212~Arg451
Tags
N-terminal His Tag
Subcellular Location
Secreted, Extracellular matrix
Traits
Freeze-dried powder
Buffer formulation
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose and Proclin300.
Predicted isoelectric point
6.9
Predicted Molecular Mass
31.3kDa
Accurate Molecular Mass
38kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Other Notes
Small volumes of MMP8 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for MMP8 recombinant protein
Positive Control, Immunogen, SDS-PAGE, WB.

(May be suitable for use in other assays to be determined by the end user.)

NCBI/Uniprot data below describe general gene information for MMP8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
matrix metalloproteinase 8 (neutrophil collagenase)
NCBI Official Synonym Full Names
matrix metallopeptidase 8
NCBI Official Symbol
NCBI Official Synonym Symbols
HNC; CLG1; MMP-8; PMNL-CL
  [Similar Products]
NCBI Protein Information
neutrophil collagenase
UniProt Protein Name
Neutrophil collagenase
UniProt Synonym Protein Names
Matrix metalloproteinase-8; MMP-8; PMNL collagenase; PMNL-CL
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CLG1; MMP-8; PMNL-CL  [Similar Products]
UniProt Entry Name
MMP8_HUMAN
NCBI Summary for MMP8
This gene encodes a member of the matrix metalloproteinase (MMP) family of proteins. These proteins are involved in the breakdown of extracellular matrix in embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Proteolysis at different sites on this protein results in multiple active forms of the enzyme with distinct N-termini. This protein functions in the degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
UniProt Comments for MMP8
MMP8: Can degrade fibrillar type I, II, and III collagens. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.34; Secreted; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: zinc ion binding; serine-type endopeptidase activity; metalloendopeptidase activity; calcium ion binding

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; proteolysis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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