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MMP9 elisa kit :: Mouse MMP-9 ELISA Kit

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Catalog # MBS175917
Unit / Price
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  96-Strip-Wells  /  $485 +1 FREE 8GB USB
 Typical Testing Data/Standard Curve (for reference only)
Product Name

MMP-9 (MMP9), ELISA Kit

Popular Item
Full Product Name

Mouse MMP-9 PicoKine ELISA Kit

Product Synonym Names
Matrix metalloproteinase-9; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); 82 kDa matrix metalloproteinase-9; 92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; Collagenase Type 4 beta; Collagenase type IV 92 KD; EC 3.4.24.35; Gelatinase 92 KD; Gelatinase B; Gelatinase beta; GelatinaseB; GELB; Macrophage gelatinase; MANDP2; Matrix metallopeptidase 9 (gelatinase B 92kDa gelatinase 92kDa type IV collangenase); Matrix metalloproteinase 9; MMP 9; MMP-9; MMP9; MMP9_HUMAN; Type V collagenase
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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3D Structure
ModBase 3D Structure for P41245
Species Reactivity
Specificity
Natural and recombinant Mouse MMP-9
Assay Type
Quantitative Sandwich
Detection Range
156pg/ml-10000pg/ml
Sensitivity
<20pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MMP9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for MMP9purchase
MBS175917 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MMP-9 (MMP9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MMP9. The ELISA analytical biochemical technique of the MBS175917 kit is based on MMP9 antibody-MMP9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MMP9 elisa kit
Principle of the Assay: Mouse MMP-9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MMP-9 has been precoated onto 96-well plates. Standards(Expression system for standard: CHO; Immunogen sequence: A20-P730) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse MMP-9 amount of sample captured in plate.

Background: The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9(MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases.1 The matrix metalloproteinases(MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.

Typical Testing Data/Standard Curve (for reference only) of MMP9 elisa kit
MMP9 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS175917. Click to request current manual
NCBI/Uniprot data below describe general gene information for MMP9. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
80,535 Da
NCBI Official Full Name
Matrix metalloproteinase-9
NCBI Official Synonym Full Names
matrix metallopeptidase 9
NCBI Official Symbol
NCBI Official Synonym Symbols
Clg4b; MMP-9; B/MMP9; AW743869; pro-MMP-9
  [Similar Products]
NCBI Protein Information
matrix metalloproteinase-9; GELB; Gel B; gelatinase B; 92kD gelatinase; 92kDa gelatinase; 92 kDa gelatinase; collagenase type IVB; 92kD type IV collagenase; 92kDa type IV collagenase; 92 kDa type IV collagenase; matrix metalloproteinase 9
UniProt Protein Name
Matrix metalloproteinase-9
UniProt Synonym Protein Names
92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Clg4b; MMP-9; GELB  [Similar Products]
UniProt Entry Name
MMP9_MOUSE
UniProt Comments for MMP9
MMP9: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. Produced by normal alveolar macrophages and granulocytes. Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1. Belongs to the peptidase M10A family.

Protein type: Protease; Secreted; Motility/polarity/chemotaxis; EC 3.4.24.35; Secreted, signal peptide

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; protein complex; extracellular region

Molecular Function: peptidase activity; identical protein binding; zinc ion binding; hydrolase activity; metallopeptidase activity; fibronectin binding; metal ion binding; metalloendopeptidase activity; endopeptidase activity; protein complex binding

Biological Process: positive regulation of keratinocyte migration; response to drug; ossification; extracellular matrix organization and biogenesis; positive regulation of apoptosis; positive regulation of leukocyte migration; tissue remodeling; positive regulation of synaptic plasticity; proteolysis; protein oligomerization; collagen catabolic process; positive regulation of angiogenesis; negative regulation of fibroblast proliferation; response to oxidative stress; leukocyte migration; skeletal development; embryo implantation; transformation of host cell by virus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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