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anti-ADAM17 antibody :: Mouse A Disintegrin And Metalloprotease 17 Monoclonal Antibody

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Catalog # MBS2025765 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $90 +1 FREE 8GB USB
  0.02 mg  /  $100 +1 FREE 8GB USB
  0.05 mg  /  $130 +1 FREE 8GB USB
  0.1 mg  /  $160 +1 FREE 8GB USB
  0.2 mg  /  $200 +1 FREE 8GB USB
  1 mg  /  $430 +1 FREE 8GB USB
Product Name

A Disintegrin And Metalloprotease 17 (ADAM17), Monoclonal Antibody

Popular Item
Full Product Name

Monoclonal Antibody to A Disintegrin And Metalloprotease 17 (ADAM17)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
U86755 mRNA
3D Structure
ModBase 3D Structure for P78536
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
C10
Host
Mouse
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1 mg/ml (lot specific)
Organism Species
Homo sapiens (Human)
Source
Monoclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant ADAM17 (Lys226~Arg473) expressed in E.coli
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-ADAM17 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ADAM17 antibody
Western Blot, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP).
Application Notes for anti-ADAM17 antibody
Western blotting: 0.5-2 ug/mL;1:500-2000
Immunohistochemistry: 5-20 ug/mL;1:50-200
Immunocytochemistry: 5-20 ug/mL;1:50-200

Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-ADAM17 antibody
Western Blot: Sample: Human U937 Cells.
anti-ADAM17 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-ADAM17 antibody
Western Blot: Sample: Recombinant ADAM17, Human.
anti-ADAM17 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ADAM17 antibody
DAB staining on IHC-P
Samples: Human Skin cancer Tissue
Primary Ab: 20 ug/ml Mouse Anti? Human
ADAM17 Antibody
Second Ab: 2 ug/mL HRP-Linked Caprine
Anti-Mouse IgG Polyclonal
Antibody (Catalog: MBS2086035)
anti-ADAM17 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ADAM17. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
disintegrin and metalloproteinase domain-containing protein 17 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 17
NCBI Official Symbol
ADAM17  [Similar Products]
NCBI Official Synonym Symbols
CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1
  [Similar Products]
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 17
UniProt Protein Name
Disintegrin and metalloproteinase domain-containing protein 17
UniProt Synonym Protein Names
Snake venom-like protease; TNF-alpha convertase; TNF-alpha-converting enzyme; CD_antigen: CD156b
UniProt Gene Name
ADAM17  [Similar Products]
UniProt Synonym Gene Names
CSVP; TACE; ADAM 17  [Similar Products]
NCBI Summary for ADAM17
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature protease. The encoded protease functions in the ectodomain shedding of tumor necrosis factor-alpha, in which soluble tumor necrosis factor-alpha is released from the membrane-bound precursor. This protease also functions in the processing of numerous other substrates, including cell adhesion proteins, cytokine and growth factor receptors and epidermal growth factor (EGF) receptor ligands. The encoded protein also plays a prominent role in the activation of the Notch signaling pathway. Elevated expression of this gene has been observed in specific cell types derived from psoriasis, rheumatoid arthritis, multiple sclerosis and Crohn's disease patients, suggesting that the encoded protein may play a role in autoimmune disease. [provided by RefSeq, Feb 2016]
UniProt Comments for ADAM17
TACE: a type I membrane protein with disintegrin and metalloprotease activity. An ubiquitously expressed ectoenzyme of peptidase family M12B. Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Possesses a narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala-|-Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha) to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, and the amyloid precursor protein. Also involved in the activation of Notch pathway. Binds 1 zinc ion per subunit. Two splice variant isoforms have been described.

Protein type: EC 3.4.24.86; Membrane protein, integral; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: actin cytoskeleton; apical plasma membrane; cell surface; cytoplasm; cytosol; focal adhesion; integral component of plasma membrane; intercellular junction; membrane; membrane raft; plasma membrane

Molecular Function: integrin binding; interleukin-6 receptor binding; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; Notch binding; PDZ domain binding; protein binding; SH3 domain binding

Biological Process: B cell differentiation; cell adhesion; cell adhesion mediated by integrin; cell motility; defense response to Gram-positive bacterium; epidermal growth factor receptor signaling pathway; germinal center formation; membrane protein ectodomain proteolysis; membrane protein intracellular domain proteolysis; negative regulation of transforming growth factor beta receptor signaling pathway; neutrophil mediated immunity; Notch receptor processing; Notch signaling pathway; PMA-inducible membrane protein ectodomain proteolysis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cellular component movement; positive regulation of chemokine production; positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle; positive regulation of epidermal growth factor-activated receptor activity; positive regulation of leukocyte chemotaxis; positive regulation of protein phosphorylation; positive regulation of T cell chemotaxis; positive regulation of transforming growth factor beta receptor signaling pathway; proteolysis; regulation of mast cell apoptotic process; response to drug; response to high density lipoprotein stimulus; response to hypoxia; response to lipopolysaccharide; spleen development; T cell differentiation in thymus; tumor necrosis factor-mediated signaling pathway; wound healing, spreading of epidermal cells

Disease: Inflammatory Skin And Bowel Disease, Neonatal, 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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