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anti-ATM antibody :: Mouse ATM Monoclonal Antibody

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Catalog # MBS190053
Unit / Price
  0.1 mg  /  $410 +1 FREE 8GB USB
anti-ATM antibody
Product Name

ATM, Monoclonal Antibody

Full Product Name

ATM Monoclonal Antibody

Product Synonym Names
Anti-ATM; ATM
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 11; NC_000011.9 (108093559..108239826). Location: 11q22-q23
OMIM
208900
3D Structure
ModBase 3D Structure for Q13315
Clonality
Monoclonal
Isotype
IgG1 kappa
Host
Mouse
Specificity
Anti-ATM [Murine MAb recognizes full-length ATM, a 370 kDa nuclear phosphoprotein associated with cell cycle regulation and response to DNA repair.]
This antibody recognizes full-length ATM, a 370 kD nuclear phosphoprotein, which is involved in the autooal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigations.
Form/Format
100 ug Protein G purified antibody in PBS, pH 7.4
Antigen
GST fusion protein expressedin E. coli corresponding to aa 2577-3056 of full-length ATM.
Stabilizer
None
Preservatives
None. Available on request.
Dilutions Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -70 degree C. Avoid multiple freeze thaw cycles.
Other Notes
Small volumes of anti-ATM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-ATM antibody
Applications Tested/Suitable for anti-ATM antibody
Immunoblot, Immunoprecipitation
Application Notes for anti-ATM antibody
Immunoblotting: use at 1-10 ug/ml.
Immunoprecipitation: use at 1-10 ug/ml.
Positive controls: Raji or Akata cells.
NCBI/Uniprot data below describe general gene information for ATM. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
350,714 Da[Similar Products]
NCBI Official Full Name
ATM
NCBI Official Synonym Full Names
ataxia telangiectasia mutated
NCBI Official Symbol
NCBI Official Synonym Symbols
AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1; MGC74674; DKFZp781A0353
  [Similar Products]
NCBI Protein Information
serine-protein kinase ATM; AT mutated; A-T mutated; OTTHUMP00000232981; TEL1, telomere maintenance 1, homolog
UniProt Protein Name
Serine-protein kinase ATM
UniProt Synonym Protein Names
Ataxia telangiectasia mutated
Protein Family
UniProt Gene Name
UniProt Entry Name
ATM_HUMAN
NCBI Summary for ATM
The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq]
UniProt Comments for ATM
ATM: an atypical kinase of the PIKK family. Regulates cell cycle checkpoints and DNA repair . May function as a tumor suppressor. Activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Involved in the activation of ABL1 and SAPK. Binds DNA ends and is part of the BRCA1- associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. DNA damage promotes association with RAD17. LOF mutations associated with ataxia telangiectasia, causing progressive loss of motor control (ataxia), dilation of superficial blood vessels (telangiectasia), cancer and immune deficiency. Approximately 30% of cases develop tumors, mostly lymphomas and leukemias, due to defects in DNA damage repair. Somatic mutations seen in leukemias and lymphomas.

Protein type: Kinase, protein; DNA repair, damage; Tumor suppressor; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical; EC 2.7.11.1; ATYPICAL group; PIKK family

Chromosomal Location of Human Ortholog: 11q22-q23

Cellular Component: nucleoplasm; chromosome, telomeric region; cytoplasmic membrane-bound vesicle; spindle

Molecular Function: protein dimerization activity; protein serine/threonine kinase activity; protein binding; DNA binding; 1-phosphatidylinositol-3-kinase activity; protein complex binding; protein N-terminus binding; DNA-dependent protein kinase activity; histone serine kinase activity; ATP binding

Biological Process: lipoprotein catabolic process; DNA damage induced protein phosphorylation; positive regulation of apoptosis; heart development; protein amino acid autophosphorylation; pre-B cell allelic exclusion; negative regulation of B cell proliferation; signal transduction; protein amino acid phosphorylation; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; double-strand break repair; positive regulation of neuron apoptosis; mitotic cell cycle spindle assembly checkpoint; cell cycle arrest; telomere maintenance; somitogenesis; V(D)J recombination; DNA repair; double-strand break repair via homologous recombination; neuron apoptosis; peptidyl-serine phosphorylation; DNA damage response, signal transduction resulting in induction of apoptosis; meiotic recombination; response to hypoxia; positive regulation of DNA damage response, signal transduction by p53 class mediator; response to ionizing radiation; brain development; response to DNA damage stimulus; oocyte development

Disease: Ataxia-telangiectasia; Breast Cancer
Product References and Citations for anti-ATM antibody
Chen, G and EY-HP Lee (1996) J Biol Chem 271: 33693-33697.

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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