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anti-BRCA1 antibody :: Mouse BRCA1 Monoclonal Antibody

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Catalog # MBS190077
Unit / Price
  0.1 mg  /  $410 +1 FREE 8GB USB
anti-BRCA1 antibody
Product Name

BRCA1, Monoclonal Antibody

Popular Item
Also Known As

BRCA1 Monoclonal Antibody

Product Synonym Names
Anti-BRCA1; BRCA1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1863
Chromosome Location
Chromosome: 17; NC_000017.10 (41196312..41277500, complement). Location: 17q21
OMIM
113705
3D Structure
ModBase 3D Structure for P38398
Clonality
Monoclonal
Isotype
IgG1 kappa
Host
Mouse
Specificity
Anti-BRCA1 [Murine MAb recognizes full-length BRCA1, a 220 kDa nuclear phosphoprotein.]
This antibody recognizes full-length BRCA1, a 220 kD nuclear phosphoprotein. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation.
Form/Format
100 ug Protein G purified antibody in PBS, pH 7.4
Antigen
GST fusion protein expressedin E. coli corresponding to aa 341-748of full-length BRCA1.
Stabilizer
None
Preservatives
None. Available on request.
Dilutions Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -70 degree C. Avoid multiple freeze thaw cycles.
Other Notes
Small volumes of anti-BRCA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-BRCA1 antibody
Applications Tested/Suitable for anti-BRCA1 antibody
Immunoblot, Immunoprecipitation
Application Notes for anti-BRCA1 antibody
Immunoblotting: use at 1-10 ug/ml.
Immunoprecipitation: use at 1-10 ug/ml.
Positive controls: Any normal human tissue, especially those containing rapidly proliferating cells, such aslymphoid germinal centers.
NCBI/Uniprot data below describe general gene information for BRCA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
207,721 Da[Similar Products]
NCBI Official Full Name
BRCA1
NCBI Official Synonym Full Names
breast cancer 1, early onset
NCBI Official Symbol
BRCA1  [Similar Products]
NCBI Official Synonym Symbols
IRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1
  [Similar Products]
NCBI Protein Information
breast cancer type 1 susceptibility protein; OTTHUMP00000212143; OTTHUMP00000212147; OTTHUMP00000212148; OTTHUMP00000212149; OTTHUMP00000212150; OTTHUMP00000212151; OTTHUMP00000212155; RING finger protein 53; BRCA1/BRCA2-containing complex, subunit 1; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein
UniProt Protein Name
Breast cancer type 1 susceptibility protein
UniProt Synonym Protein Names
RING finger protein 53
Protein Family
UniProt Gene Name
BRCA1  [Similar Products]
UniProt Synonym Gene Names
RNF53  [Similar Products]
UniProt Entry Name
BRCA1_HUMAN
NCBI Summary for BRCA1
This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq]
UniProt Comments for BRCA1
BRCA1: the BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACC1 and preventing its dephosphorylation. Defects in BRCA1 are a cause of genetic susceptibility to breast cancer. Mutations in BRCA1 are thought to be responsible for more than 80% of inherited breast-ovarian cancer. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association may be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts (via BRCT domains) with CTIP. Associates with RNA polymerase II holoenzyme. Interacts with SMC1 and COBRA1. Interacts (via BRCT domains) with BRIP1. Interacts with FANCD2 (ubiquitinated). Interacts with BAP1. Interacts with Artemis and claspin. Interacts with H2AFX (phosphorylated on S140). Interacts with CHK1. Interacts with BRCC3. Five human isoforms are produced by alternative splicing and alternative initiation. Isoform 1 and isoform 3 are widely expressed. Isoform 1 is largely nuclear, while isoforms 3 and 5 are cytoplasmic.

Protein type: Nuclear receptor co-regulator; Tumor suppressor; Transcription, coactivator/corepressor; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; gamma-tubulin ring complex; protein complex; condensed nuclear chromosome; BRCA1-BARD1 complex; cytoplasm; plasma membrane; chromosome; ribonucleoprotein complex; nucleus; ubiquitin ligase complex

Molecular Function: tubulin binding; protein binding; enzyme binding; androgen receptor binding; DNA binding; zinc ion binding; RNA binding; ubiquitin protein ligase binding; transcription coactivator activity; ubiquitin-protein ligase activity; damaged DNA binding; ligase activity

Biological Process: genetic imprinting; apoptosis; positive regulation of transcription, DNA-dependent; dosage compensation, by inactivation of X chromosome; centrosome cycle; protein ubiquitination; negative regulation of histone H3-K4 methylation; positive regulation of histone H3-K4 methylation; chromosome segregation; negative regulation of histone acetylation; regulation of apoptosis; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; double-strand break repair; DNA replication; negative regulation of fatty acid biosynthetic process; fatty acid biosynthetic process; positive regulation of histone H3-K9 methylation; negative regulation of histone H3-K9 methylation; protein autoubiquitination; chordate embryonic development; positive regulation of DNA repair; postreplication repair; DNA repair; negative regulation of centriole replication; double-strand break repair via homologous recombination; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; positive regulation of angiogenesis; DNA damage response, signal transduction resulting in induction of apoptosis; regulation of transcription from RNA polymerase III promoter; positive regulation of protein ubiquitination; response to estrogen stimulus; androgen receptor signaling pathway; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; response to ionizing radiation; negative regulation of transcription, DNA-dependent; G2/M transition DNA damage checkpoint; response to DNA damage stimulus

Disease: Breast-ovarian Cancer, Familial, Susceptibility To, 1; Pancreatic Cancer, Susceptibility To, 4; Breast Cancer
Product References and Citations for anti-BRCA1 antibody
Chen, Y et al. (1995) Science 270: 789-791; Chen, C-F et al. (1995) J Biol Chem 271: 32863-32868; Chen, Y et al. (1996) Cancer Res 56: 3168-3172.

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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