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anti-C4 antibody :: Rat anti-C4b
C4d C4 Monoclonal Antibody

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Catalog # MBS584166
Unit / Price
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  0.1 mg  /  $610 +1 FREE 8GB USB
anti-C4 antibody
Product Name

C4, Monoclonal Antibody

Popular Item
Full Product Name

C4, Mouse, mAb 16D2

Product Synonym Names
Complement component 4
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P01029
Clonality
Monoclonal
Isotype
Rat IgG2a
Host
Rat
Species Reactivity
C4b
C4d
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Immunogen
Thymocytes decorated with Thy-1 antibody and complement components
Positive Control
T-cell regions of mouse spleen
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Other Notes
Small volumes of anti-C4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-C4 antibody
Product Description: The monoclonal antibody 16D2 recognizes mouse complement factor C4, formerly known as Gg protein, which consists of an alpha-, beta-, and gamma-chain. The classical pathway of complement and the Mannose binding lectin activation pathway converge at C4. C1s, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. Subsequently, C4b can be cleaved to C4c and C4d by other serum enzymes. The monoclonal antibody 16D2 reacts with intact C4, C4b and C4d.
C4 is an acute phase protein that is produced by hepatocytes, monocytes and intestinal epithelial cells and can be used- in experimental animals as a marker for activation of the classical complement pathway. Recent studies have demonstrated an association between graft rejection and C4d deposition in a mouse model for cardiac transplantation.
Applications Tested/Suitable for anti-C4 antibody
Immunoassay (IA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-C4 antibody
Application Notes: W: A non-reduced sample treatment and SDS-Page was used. The band size is 200x kDa (Ref.4).
IA: Plates were coated overnight with rat anti-murine C4 mAb 16D2 in carbonate buffer and blocked with 5% dry milk in PBS and 0.01% Tween 20.
IF: Spleens were snap frozen and stored at -80 degree C until cryosections were cute. (Section (5um) were fixed for 4 mins in ice-cold acetone. Sections were blocked with 2% BSA, 2% FCS and PBS. Biotinylated 16D2 was visualized using avidin-PE.
Application Use: For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
NCBI/Uniprot data below describe general gene information for C4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
192,915 Da
NCBI Official Full Name
C4, partial
NCBI Official Synonym Full Names
complement component 4B (Chido blood group)
NCBI Official Symbol
NCBI Official Synonym Symbols
C4; Ss
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NCBI Protein Information
complement C4-B; complement component 4 (within H-2S); complement component 4B (Childo blood group)
UniProt Protein Name
Complement C4-B
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
CO4B_MOUSE
UniProt Comments for C4
C4B: C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway. Defects in C4B are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Interindividual copy- number variation (CNV) of complement component C4 and associated polymorphisms result in different susceptibilities to SLE. The risk of SLE susceptibility has been shown to be significantly increased among subjects with only two copies of total C4. A high copy number is a protective factor against SLE. Defects in C4B are the cause of complement component 4B deficiency (C4BD). A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: complement binding; complement component C1q binding; carbohydrate binding

Biological Process: immunoglobulin mediated immune response; complement activation
Product References and Citations for anti-C4 antibody
1. Kremmer, E et al; Monoclonal antibodies to complement components without the need of their prior purification. II. Antibodies to mouse C3 and C4. Hybridoma 1990, 9 : 309 2. Gadjeva, M et al; Macrophage-derived complement component C4 can restore humoral immunity in C4-deficient mice. J Immunol 2002, 169: 5489
3. Paul E et al; Anti-DNA autoreactivity in C4-deficient mice. Eur J Immunol 2002, 32: 2672
4. Murata, K et al; Synergistic deposition of C4d by complement-activating and non-activating antibodies in cardiac transplants. Am J Transplant 2007, 7: 2605

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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