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anti-CD117 antibody :: Mouse CD117 Monoclonal Antibody

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Catalog # MBS215263
Unit / Price
  25 Tests  /  $200 +1 FREE 8GB USB
anti-CD117 antibody
Product Name

CD117, Monoclonal Antibody

Also Known As

MOUSE ANTI HUMAN CD117:RPE

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
976
OMIM
164920
3D Structure
ModBase 3D Structure for P10721
Clonality
Monoclonal
Isotype
IgG1
Clone Number
104D2
Host
Mouse
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Perservative Stabilisers
0.09% Sodium Azide
0.2% Bovine Serum Albumin
Immunogen
MOLM-1 megakaryocytic cell line.
Buffer Solution
Target Species
Human
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 6 months from date of despatch.
Other Notes
Small volumes of anti-CD117 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD117 antibody
Mouse anti Human CD117 antibody, clone 104D2 recognizes the human CD117 cell surface antigen, a 145kDa glycoprotein also known as c-kit. CD117 is expressed by some haematopoietic stem cells (approx. 70% of CD34+ve cells in normal bone marrow are CD117+ve), some acute myeloid leukaemias and by mast cells. Functionally CD117 is a receptor for stem cell factor, and has receptor tyrosine kinase activity. Mouse anti Human CD117 antibody, clone 104D2 is non-neutralising.
Applications Tested/Suitable for anti-CD117 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD117 antibody
Flow Cytometry: Use 20 ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat
NCBI/Uniprot data below describe general gene information for CD117. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
46,658 Da
NCBI Official Full Name
mast/stem cell growth factor receptor Kit isoform 1
NCBI Official Synonym Full Names
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
NCBI Official Symbol
NCBI Official Synonym Symbols
PBT; SCFR; C-Kit; CD117
  [Similar Products]
NCBI Protein Information
mast/stem cell growth factor receptor Kit; p145 c-kit; piebald trait protein; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; soluble KIT variant 1; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
UniProt Protein Name
Mast/stem cell growth factor receptor Kit
UniProt Synonym Protein Names
Piebald trait protein; PBT; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD_antigen: CD117
UniProt Gene Name
UniProt Synonym Gene Names
SCFR; SCFR; PBT  [Similar Products]
UniProt Entry Name
KIT_HUMAN
NCBI Summary for CD117
This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for CD117
Kit: a receptor tyrosine kinase and a member of the subfamily that includes PDGF, CSF-1 and FLT-3/flk-2 receptors. Receptor for stem cell factor. Plays a critical role in hematopoietic stem cell, mast cell, melanocyte and germ cell development. Ligand binding induces autophosphorylation, dimerization and activation, leading to the recruitment and phosphorylation of downstream SH2-containing signaling components including PLC-gamma, PI3 kinase p85, SHP2 and CrkL, linking c-Kit to various cell signaling pathways. Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders, while mutations that constitutively activate c-Kit can lead to hyperplasia and tumorigenesis. Activating mutations cause >90% of gastrointestinal stromal tumors (GIST); successfully treated with inhibitors Gleevec (imatinib, Glivec) and Sutent (Sutinib, SU11248). Activating mutations also induce mastocytosis. Autocrine/paracrine stimulation may drive some lung and other tumors. Loss of expression associated with melanoma progression. Familial loss of function mutations cause piebaldism, with defects in hair and skin pigmentation due to lack of melanocytes.

Protein type: Protein kinase, TK; Membrane protein, integral; Oncoprotein; EC 2.7.10.1; Kinase, protein; Protein kinase, tyrosine (receptor); TK group; PDGFR family

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: extracellular space; internal side of plasma membrane; mast cell granule; plasma membrane; integral to membrane; acrosome; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; protease binding; protein-tyrosine kinase activity; metal ion binding; cytokine binding; stem cell factor receptor activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: nerve growth factor receptor signaling pathway; somatic stem cell maintenance; activation of MAPK activity; germ cell programmed cell death; positive regulation of JAK-STAT cascade; lymphoid progenitor cell differentiation; positive regulation of long-term neuronal synaptic plasticity; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of cell shape; epithelial cell proliferation; germ cell migration; erythrocyte differentiation; somatic stem cell division; T cell differentiation; fibroblast growth factor receptor signaling pathway; embryonic hemopoiesis; mast cell chemotaxis; stem cell differentiation; detection of mechanical stimulus involved in sensory perception of sound; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of tyrosine phosphorylation of Stat1 protein; regulation of pigmentation during development; glycosphingolipid metabolic process; immature B cell differentiation; positive regulation of transcription factor activity; mast cell cytokine production; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; signal transduction; positive regulation of MAPKKK cascade; myeloid progenitor cell differentiation; ovarian follicle development; melanocyte differentiation; positive regulation of cell proliferation; negative regulation of programmed cell death; visual learning; hemopoiesis; inflammatory response; positive regulation of Notch signaling pathway; epidermal growth factor receptor signaling pathway; lamellipodium biogenesis; phosphoinositide-mediated signaling; dendritic cell cytokine production; cytokine and chemokine mediated signaling pathway; male gonad development; stem cell maintenance; positive regulation of phosphoinositide 3-kinase activity; mast cell degranulation; regulation of cell proliferation; positive regulation of pseudopodium formation; pigmentation; positive regulation of tyrosine phosphorylation of Stat5 protein; gut development; actin cytoskeleton reorganization; innate immune response; spermatogenesis; spermatid development; positive regulation of cell migration

Disease: Gastrointestinal Stromal Tumor; Mast Cell Disease; Piebald Trait; Testicular Germ Cell Tumor
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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